微型中空纤维辅助液相微萃取结合原位衍生化及气相色谱-质谱联用分析人尿样中的双酚A
Miniaturized hollow fiber assisted liquid-phase microextraction with in situ derivatization and gas chromatography-mass spectrometry for analysis of bisphenol A in human urine sample.
作者信息
Kawaguchi Migaku, Ito Rie, Okanouchi Noriya, Saito Koichi, Nakazawa Hiroyuki
机构信息
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Tokyo 142-8501, Japan.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jul 1;870(1):98-102. doi: 10.1016/j.jchromb.2008.06.011. Epub 2008 Jun 13.
A new method that involves miniaturized hollow fiber assisted liquid-phase microextraction (HF-LPME) with in situ derivatization and gas chromatography-mass spectrometry (GC-MS) is described for the determination of trace amounts of bisphenol A (BPA) in human urine samples. The detection limit and the quantification limit of BPA in human urine sample are 0.02 and 0.1 ng ml(-1) (ppb), respectively. The calibration curve for BPA is linear with a correlation coefficient of >0.999 in the range of 0.1-50 ng ml(-1). The average recoveries of BPA in human urine samples spiked with 1 and 5 ng ml(-1) BPA are 101.0 (R.S.D.: 6.7%) and 98.8 (R.S.D.: 1.8%), respectively, with correction using the added surrogate standard, bisphenol A-(13)C12. This simple, accurate, sensitive and selective analytical method can be applicable to the determination of trace amounts of BPA in human urine samples.
本文描述了一种新方法，该方法涉及采用原位衍生化的小型中空纤维辅助液相微萃取（HF-LPME）和气相色谱-质谱联用（GC-MS）技术，用于测定人尿液样本中的痕量双酚A（BPA）。人尿液样本中BPA的检测限和定量限分别为0.02和0.1 ng ml⁻¹（ppb）。BPA的校准曲线呈线性，在0.1 - 50 ng ml⁻¹范围内相关系数>0.999。添加1和5 ng ml⁻¹ BPA的人尿液样本中BPA的平均回收率分别为101.0（相对标准偏差：6.7%）和98.8（相对标准偏差：1.8%），使用添加的替代标准双酚A-(¹³)C₁₂进行校正。这种简单、准确、灵敏且具选择性的分析方法可适用于测定人尿液样本中的痕量BPA。
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