Kawaguchi Migaku, Ito Rie, Endo Naoyuki, Okanouchi Noriya, Sakui Norihiro, Saito Koichi, Nakazawa Hiroyuki
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
J Chromatogr A. 2006 Mar 31;1110(1-2):1-5. doi: 10.1016/j.chroma.2006.01.061. Epub 2006 Feb 3.
A new method that involves liquid phase microextraction (LPME) with in situ derivatization and gas chromatography-mass spectrometry (GC-MS) is described for the determination of trace amounts of bisphenol A (BPA) in river water samples. The LPME conditions, such as the type of extraction solvent and the extraction time, are investigated. Then, the extract is directly injected into GC-MS. The detection limit and the quantification limit of BPA in river water sample are 2 and 10pgml(-1) (ppt), respectively. The calibration curve for BPA is linear with a correlation coefficient of >0.999 in the range of 10-10,000pgml(-1). The average recoveries of BPA in river water samples spiked with 100 and 1000pgml(-1) BPA are 104.1 (RSD: 8.9%) and 98.3 (RSD: 3.2%), respectively, with correction using the added surrogate standard, bisphenol A-(13)C(12). This simple, accurate, sensitive and selective analytical method may be applicable to the determination of trace amounts of BPA in liquid samples.
描述了一种新方法，该方法涉及采用原位衍生化的液相微萃取（LPME）和气相色谱-质谱联用（GC-MS）来测定河水样品中的痕量双酚A（BPA）。研究了LPME条件，如萃取溶剂类型和萃取时间。然后，将萃取物直接注入GC-MS。河水样品中BPA的检测限和定量限分别为2和10 pg ml⁻¹（ppt）。BPA的校准曲线呈线性，在10 - 10000 pg ml⁻¹范围内相关系数>0.999。添加100和1000 pg ml⁻¹ BPA的河水样品中BPA的平均回收率分别为104.1（相对标准偏差：8.9%）和98.3（相对标准偏差：3.2%），使用添加的替代标准双酚A-(¹³)C(¹²)进行校正。这种简单、准确、灵敏且具有选择性的分析方法可能适用于测定液体样品中的痕量BPA。
