[慢性阻塞性肺疾病患者的氧化应激状态及其与糖皮质激素受体水平的关系]

[Oxidative stress status in patients with chronic obstructive pulmonary disease and its relation to glucocorticoid receptor levels].

作者信息

Liu Ling-Yun, Zeng Mian, Xie Can-Mao, Gao Jing-Hui, Yan Ying-Shuo, Lu Gui-Fang, Wang Hui, He Yun-Peng

机构信息

Department of Respiratory Diseases, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2008 Jun;28(6):992-6.

PMID:18583246

Abstract

OBJECTIVE

To study changes in the levels of systematic and airway local oxidative stress in patients in different stages of chronic obstructive pulmonary diseases (COPD), and explore the association between oxidative stress and glucocorticoid receptor (GR) level in the peripheral blood leukocytes.

METHODS

The levels of malonaldehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) in induced sputum and plasma, as well as GR levels in peripheral blood leukocytes and plasma levels of cortisol and adrenocorticotrophic hormone (ACTH), were examined in 33 patients with acute exacerbations of COPD (AECOPD, group A), 27 with stable COPD (group B), and 28 healthy volunteers (including 15 smokers as group C, and 15 nonsmokers as group D).

RESULTS

MDA level in induced sputum and plasma decreased, whereas the levels of GSH, SOD and GSH-PX increased significantly in the order of groups A, B, C, and D (P<0.05). The activity of SOD in induced sputum and plasma were significantly lower in group C than in group D. No significant difference was noted in the other oxidative stress indices between groups C and D (P>0.05). The plasma levels of cortisol and ACTH showed no significant difference between the 4 groups, while the GR level in peripheral blood leukocytes increased significantly in the order of groups A, B, C and D (1565-/+719, 2069-/+488, 2739-/+926, and 4793 -/+1415 U, respectively, P<0.05). After controlling for the factor of smoking status, the plasma and sputum SOD activity were both positively correlated to GR, with the partial correlation coefficient of 0.512 and 0.564, respectively (P<0.001).

CONCLUSION

Patients in different stages of COPD, especially those with AECOPD, may sustain systematic and local oxidation and anti-oxidation imbalance. Decreased SOD activity may contribute to GR level decrement in peripheral blood leukocytes in these patients.

摘要

目的

研究慢性阻塞性肺疾病（COPD）不同阶段患者全身及气道局部氧化应激水平的变化，探讨氧化应激与外周血白细胞糖皮质激素受体（GR）水平之间的关系。

方法

检测33例慢性阻塞性肺疾病急性加重期（AECOPD，A组）患者、27例稳定期COPD患者（B组）及28例健康志愿者（包括15例吸烟者为C组，15例不吸烟者为D组）诱导痰及血浆中丙二醛（MDA）、谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）水平，以及外周血白细胞GR水平和血浆皮质醇及促肾上腺皮质激素（ACTH）水平。

结果

诱导痰及血浆中MDA水平降低，而GSH、SOD和GSH-PX水平按A、B、C、D组顺序显著升高（P<0.05）。C组诱导痰及血浆中SOD活性显著低于D组。C组和D组其他氧化应激指标差异无统计学意义（P>0.05）。4组间血浆皮质醇及ACTH水平差异无统计学意义，而外周血白细胞GR水平按A、B、C、D组顺序显著升高（分别为1565±719、2069±488、2739±926和4793±1415 U，P<0.05）。控制吸烟状态因素后，血浆和痰中SOD活性均与GR呈正相关，偏相关系数分别为0.512和0.564（P<0.001）。