Gumral Nurhan, Naziroglu Mustafa, Ongel Kurtulus, Beydilli Emine Dasdibi, Ozguner Fehmi, Sutcu Recep, Caliskan Sadettin, Akkaya Ahmet
Department of Physiology, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkey.
Cell Biochem Funct. 2009 Jul;27(5):276-83. doi: 10.1002/cbf.1569.
An imbalance between oxidative stress and antioxidative capacity may play an important role in the development and progression of bronchial asthma (BA) and chronic obstructive pulmonary disease (COPD). We carried out a study to assess the systemic oxidant-antioxidant status during the exacerbation and the stable period in patients with BA and COPD. A total of 33 patients, 16 with BA and 17 with COPD were included in the study. During the exacerbation and the stable periods, levels of malondialdehyde (MDA), activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione reductase (GRd), and catalase (CAT) in erythrocytes and serum melatonin concentrations were investigated. Blood counts, respiratory functions, and blood gases of the patients were also performed. During an exacerbation period of BA, despite the decreases in GSH-Px, GRd and melatonin levels, MDA and CAT levels, and the white blood cell count, the percentage of eosinophils were significantly higher than in the stable period. Also, it was found that FEV(1)/L (where FEV(1) is the forced expiratory volume in 1 s), FVC/L (where FVC is forced vital capacity), PEF/L/s (where PEF is peak expiratory flow), pO(2) (where pO(2) is oxygen pressure) levels increased during the stable period in patients with BA. MDA and SOD values were higher in the exacerbation period than in the stable period although GSH-Px, GRd, melatonin, pH, and pO(2) values were lower in the exacerbation period than in the stable period. The blood counts and the respiratory function tests did not change between the exacerbation and the stable period of patients with COPD significantly. In conclusion, we observed that oxidative stress in the exacerbation period of patients with BA and COPD increased whereas the antioxidant enzymes and melatonin values reduced. The episodes of BA or COPD might be associated with elevated levels of oxidative stress.
氧化应激与抗氧化能力之间的失衡可能在支气管哮喘（BA）和慢性阻塞性肺疾病（COPD）的发生和发展中起重要作用。我们开展了一项研究，以评估BA和COPD患者病情加重期和稳定期的全身氧化-抗氧化状态。该研究共纳入33例患者，其中16例为BA患者，17例为COPD患者。在病情加重期和稳定期，分别检测红细胞中丙二醛（MDA）水平、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽还原酶（GRd）和过氧化氢酶（CAT）的活性以及血清褪黑素浓度。同时还对患者进行了血常规、呼吸功能和血气分析。在BA病情加重期，尽管GSH-Px、GRd和褪黑素水平降低，MDA和CAT水平以及白细胞计数下降，但嗜酸性粒细胞百分比仍显著高于稳定期。此外，还发现BA患者稳定期的1秒用力呼气容积（FEV₁）/L、用力肺活量（FVC）/L、呼气峰值流速（PEF）/L/s和氧分压（pO₂）水平升高。BA患者病情加重期的MDA和SOD值高于稳定期，而GSH-Px、GRd、褪黑素、pH和pO₂值则低于稳定期。COPD患者病情加重期和稳定期的血常规和呼吸功能测试结果无明显变化。总之，我们观察到BA和COPD患者病情加重期的氧化应激增加，而抗氧化酶和褪黑素值降低。BA或COPD的发作可能与氧化应激水平升高有关。
