从人类眼部样本中分离出的棘阿米巴菌株的基因型特征分析。
The genotypic characterisation of Acanthamoeba isolates from human ocular samples.
作者信息
Yera H, Zamfir O, Bourcier T, Viscogliosi E, Noël C, Dupouy-Camet J, Chaumeil C
机构信息
Laboratoire de Parasitologie Mycologie, Hôpital Cochin, 27 rue du Faubourg Saint Jacques, 75014 Paris, France.
出版信息
Br J Ophthalmol. 2008 Aug;92(8):1139-41. doi: 10.1136/bjo.2007.132266. Epub 2008 Jun 27.
BACKGROUND
We characterised 37 amoebae cultured from corneal scrapings, contact lenses or lens case solutions of patients with suspected Acanthamoeba keratitis.
METHODS
The isolates were identified by their morphology and by PCR targeting the Acanthamoeba nuclear small-subunit rRNA gene. Acanthamoeba isolates were genotyped by DNA sequence analysis.
RESULTS
The 37 isolates comprised 35 Acanthamoeba, one Hartmannella and one Vahlkampfia. Ten Acanthamoeba isolates were shown to be responsible for keratitis.
CONCLUSION
Genotype T4 was the only Acanthamoeba genotype responsible for keratitis in this study, and represented 79% of non-pathogenic isolates.
背景
我们对从疑似棘阿米巴角膜炎患者的角膜刮片、隐形眼镜或镜盒溶液中培养出的37株阿米巴原虫进行了特征分析。
方法
通过形态学以及针对棘阿米巴核小亚基rRNA基因的PCR对分离株进行鉴定。通过DNA序列分析对棘阿米巴分离株进行基因分型。
结果
这37株分离株包括35株棘阿米巴、1株哈特曼氏阿米巴和1株瓦氏阿米巴。10株棘阿米巴分离株被证实与角膜炎有关。
结论
在本研究中，基因型T4是唯一导致角膜炎的棘阿米巴基因型，并且在非致病性分离株中占79%。
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