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棘阿米巴介导的细胞病变效应与 MBP 和 AhLBP mRNA 表达相关。

Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression.

机构信息

Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, 56000, Kuala Lumpur, Bandar Tun Razak, Malaysia.

Department of Parasitology and Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, 56000, Kuala Lumpur, Bandar Tun Razak, Malaysia.

出版信息

Parasit Vectors. 2017 Dec 28;10(1):625. doi: 10.1186/s13071-017-2547-0.

DOI:10.1186/s13071-017-2547-0
PMID:29282148
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5745754/
Abstract

BACKGROUND

In recent years, the concern of Acanthamoeba keratitis has increased since the infection is often associated with contact lens use. Partial 18S rRNA genotypic identification of Acanthamoeba isolates is important to correlate with pathophysiological properties in order to evaluate the degree of virulence. This is the first report of genotypic identification for clinical isolates of Acanthamoeba from corneal scrapings of keratitis in Malaysia. This study is also the first to correlate the mRNA expression of MBP and AhLBP as virulent markers for axenic strains of Acanthamoeba.

RESULTS

In this study, ten clinical isolates were obtained from corneal scrapings. Rns genotype and intra-genotypic variation at the DF3 region of the isolates were identified. Results revealed that all clinical isolates belonged to the T4 genotype, with T4/6 (4 isolates), T4/2 (3 isolates), T4/16 (2 isolates) and one new genotype T4 sequence (T4/36), being determined. The axenic clinical isolates were cytopathogenic to rabbit corneal fibroblasts. MBP and AhLBP mRNA expression are directly correlated to Acanthamoeba cytopathic effect.

CONCLUSIONS

All ten Malaysian clinical isolates were identified as genotype T4 which is predominantly associated with AK. Measuring the mRNA expression of Acanthamoeba virulent markers could be useful in the understanding of the pathogenesis of Acanthamoeba keratitis.

摘要

背景

近年来，棘阿米巴角膜炎的关注度有所增加，因为这种感染通常与隐形眼镜的使用有关。棘阿米巴部分 18S rRNA 基因型的鉴定对于与病理生理特性相关联以评估毒力程度非常重要。这是马来西亚首次从角膜炎角膜刮片中报告棘阿米巴临床分离株的基因鉴定。本研究也是首次将 MBP 和 AhLBP 的 mRNA 表达与棘阿米巴的无共生菌株的毒力标记物相关联。

结果

本研究从角膜刮片中获得了 10 株临床分离株。鉴定了 Rns 基因型和分离株 DF3 区的种内变异。结果表明，所有临床分离株均属于 T4 基因型，其中 T4/6（4 株）、T4/2（3 株）、T4/16（2 株）和一个新的 T4 序列（T4/36）。无共生的临床分离株对兔角膜成纤维细胞具有细胞病变作用。MBP 和 AhLBP mRNA 的表达与棘阿米巴的细胞病变效应直接相关。

结论

所有 10 株马来西亚临床分离株均鉴定为 T4 基因型，这与 AK 密切相关。测量棘阿米巴毒力标记物的 mRNA 表达可能有助于了解棘阿米巴角膜炎的发病机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6ca/5745754/1fe0bfe0b113/13071_2017_2547_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6ca/5745754/554ca8800c0e/13071_2017_2547_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6ca/5745754/09fb9cbdab05/13071_2017_2547_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6ca/5745754/7f4aad91acf2/13071_2017_2547_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6ca/5745754/490ed6e81d5c/13071_2017_2547_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6ca/5745754/8fd70808fb22/13071_2017_2547_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6ca/5745754/216e531afd3d/13071_2017_2547_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6ca/5745754/1fe0bfe0b113/13071_2017_2547_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6ca/5745754/554ca8800c0e/13071_2017_2547_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6ca/5745754/09fb9cbdab05/13071_2017_2547_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6ca/5745754/7f4aad91acf2/13071_2017_2547_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6ca/5745754/490ed6e81d5c/13071_2017_2547_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6ca/5745754/8fd70808fb22/13071_2017_2547_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6ca/5745754/216e531afd3d/13071_2017_2547_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6ca/5745754/1fe0bfe0b113/13071_2017_2547_Fig7_HTML.jpg

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