在标准条件下用于接种形成极小噬菌斑或无噬菌斑的大肠杆菌噬菌体的简单方法。
Simple method for plating Escherichia coli bacteriophages forming very small plaques or no plaques under standard conditions.
作者信息
Loś Joanna M, Golec Piotr, Wegrzyn Grzegorz, Wegrzyn Alicja, Loś Marcin
机构信息
Department of Molecular Biology, University of Gdańsk, Kładki 24, 80-822 Gdańsk, Poland.
出版信息
Appl Environ Microbiol. 2008 Aug;74(16):5113-20. doi: 10.1128/AEM.00306-08. Epub 2008 Jun 27.
Abstract
The use of low concentrations (optimally 2.5 to 3.5 microg/ml, depending on top agar thickness) of ampicillin in the bottom agar of the plate allows for formation of highly visible plaques of bacteriophages which otherwise form extremely small plaques or no plaques on Escherichia coli lawns. Using this method, we were able to obtain plaques of newly isolated bacteriophages, propagated after induction of prophages present in six E. coli O157:H(-) strains which did not form plaques when standard plating procedures were employed.
摘要
在平板底层琼脂中使用低浓度（最佳浓度为2.5至3.5微克/毫升，具体取决于顶层琼脂的厚度）的氨苄青霉素，可使噬菌体形成高度可见的噬菌斑，否则这些噬菌体在大肠杆菌菌苔上会形成极小的噬菌斑或不形成噬菌斑。使用这种方法，我们能够获得新分离噬菌体的噬菌斑，这些噬菌体是在诱导存在于6株大肠杆菌O157:H(-)菌株中的原噬菌体后增殖得到的，而采用标准平板接种程序时这些菌株不形成噬菌斑。
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