基于噬菌体评分的液体培养基中感染鲍曼不动杆菌的噬菌体-抗生素相互作用分析。
PhageScore-based analysis of Acinetobacter baumannii infecting phages antibiotic interaction in liquid medium.
机构信息
Chair of Microbiology, Department of Laboratory Medicine, Immunology and Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstańców Wielkopolskich 72, 70-111, Szczecin, Poland.
Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Piastów Avenue 42, 71-065, Szczecin, Poland.
出版信息
Arch Microbiol. 2022 Jun 24;204(7):421. doi: 10.1007/s00203-022-03020-7.
The growing interest in bacteriophages and antibiotics' combined use poses new challenges regarding this phenomenon's accurate description. This study aimed to apply the PhageScore methodology to assess the phage-antibiotic combination activity in liquid bacterial culture. For this purpose, previously described Acinetobacter infecting phages vB_AbaP_AGC01, Aba-1, and Aba-4 and antibiotics (gentamicin, ciprofloxacin, meropenem, norfloxacin, and fosfomycin) were used to obtain a lysis curve of bacteriophages under antibiotic pressure. The experimental data were analyzed using the Fractional Inhibitory Concentration Index (FICI) and PhageScore methodology. The results obtained by this method clearly show differences between phage lytic activity after antibiotic addition. Thus, we present the potential use of the PhageScore method as a tool for characterizing the phage antibiotic synergy in liquid culture. Further, the optimization of the PhageScore for this purpose can help compare antibiotics and their outcome on bacteriophage lytic activity.
人们对噬菌体和抗生素联合使用的兴趣日益浓厚，这给准确描述这一现象带来了新的挑战。本研究旨在应用 PhageScore 方法评估液体细菌培养中噬菌体-抗生素组合的活性。为此，使用了先前描述的感染不动杆菌的噬菌体 vB_AbaP_AGC01、Aba-1 和 Aba-4 以及抗生素（庆大霉素、环丙沙星、美罗培南、诺氟沙星和磷霉素），在抗生素压力下获得噬菌体的裂解曲线。使用分数抑制浓度指数 (FICI) 和 PhageScore 方法对实验数据进行分析。该方法得到的结果清楚地显示了抗生素添加后噬菌体裂解活性的差异。因此，我们提出了 PhageScore 方法作为一种工具的潜在用途，用于在液体培养中表征噬菌体抗生素协同作用。此外，为此目的优化 PhageScore 有助于比较抗生素及其对噬菌体裂解活性的影响。
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