使用银增强金标记免疫测定法检测全血样本中的人类免疫缺陷病毒抗体。

Use of a silver-enhanced gold-labelled immunoassay for detection of antibodies to the human immunodeficiency virus in whole blood samples.

作者信息

Rocks B F, Patel N, Bailey M P

机构信息

Biochemistry Department, Royal Sussex County Hospital, Brighton, UK.

出版信息

Ann Clin Biochem. 1991 Mar;28 ( Pt 2):155-9. doi: 10.1177/000456329102800206.

DOI:10.1177/000456329102800206

PMID:1859153

Abstract

A silver-enhanced gold-labelled immunosorbent assay (SEGLISA) for the detection of antibodies to the immunodeficiency virus (HIV) in whole-blood samples is described. This new non-isotopic, non-enzymic immunoassay incorporates use of solid phase viral antigens which bind any HIV antibodies present in the test sample. The antigen/antibody complex is then detected by gold-labelled anti human immunoglobulin G (IgG) followed by silver amplification. We found that whole blood samples give false positives when using a horseradish peroxidase label, whereas the SEGLISA correctly identified 50 HIV antibody positive samples and 50 HIV antibody negative samples when using whole blood. The use of whole blood collected on filter paper is also described. The SEGLISA has good precision (CV = 7.5%) and sensitivity.

摘要

本文描述了一种用于检测全血样本中免疫缺陷病毒（HIV）抗体的银增强金标记免疫吸附测定法（SEGLISA）。这种新的非同位素、非酶免疫测定法采用固相病毒抗原，可结合测试样本中存在的任何HIV抗体。然后通过金标记的抗人免疫球蛋白G（IgG）检测抗原/抗体复合物，随后进行银增强。我们发现，使用辣根过氧化物酶标记时，全血样本会出现假阳性，而使用全血时，SEGLISA正确识别了50份HIV抗体阳性样本和50份HIV抗体阴性样本。本文还介绍了使用滤纸上采集的全血。SEGLISA具有良好的精密度（CV = 7.5%）和灵敏度。