Patel N, Rocks B F, Bailey M P
Biochemistry Department, Royal Sussex County Hospital, Brighton.
J Clin Pathol. 1991 Apr;44(4):334-8. doi: 10.1136/jcp.44.4.334.
A silver enhanced, gold labelled, immunosorbent assay (SEGLISA) for the detection of IgG antibodies to the rubella virus in human serum was developed. Pre-coated microtitre wells are used as the immobilised base of rubella antigens on to which any rubella antibodies from patient samples will bind. This antigen/antibody complex is then visualised firstly by gold labelled anti-immunoglobulin G, which binds to any human IgG that may be present, and then by silver amplification, resulting in a black permanent deposit on the microtitre well surface. Patient samples (n = 121) were screened using a commercially available enzyme linked immunosorbent assay (ELISA) and an equivalent SEGLISA. Results were comparable but the SEGLISA does not have the disadvantages associated with enzyme labels. The silver deposit may also be read visually or the dried plate may be stored for future reference.
开发了一种用于检测人血清中风疹病毒IgG抗体的银增强、金标记免疫吸附测定法（SEGLISA）。预包被的微量滴定孔用作风疹抗原的固定基质，患者样本中的任何风疹抗体都会与之结合。然后，首先通过与可能存在的任何人IgG结合的金标记抗免疫球蛋白G使这种抗原/抗体复合物可视化，接着通过银增强，在微量滴定孔表面产生黑色永久性沉淀物。使用市售酶联免疫吸附测定法（ELISA）和等效的SEGLISA对121份患者样本进行筛查。结果具有可比性，但SEGLISA没有与酶标记相关的缺点。银沉淀物也可以目视读取，或者干燥的平板可以保存以备将来参考。
