Kehayov I R
Ann Immunol (Paris). 1976 Sep-Oct;127(5):703-16.
A rabbit antiserum to human glioblastoma is tested against extracts from normal adult human organs and brain tumors. By agarose-gel immunodiffusion it is established that along with glioblastoma extract this antiserum reacts also against extracts from human kidney, lung, liver, spleen, testis and normal brain. After complete absorption with lyophilized pooled human blood plasma and consequently with extracts from normal organs (including normal brain) it continues to react with the glioblastoma extract only. This completely absorbed anti-glioblastoma antiserum gives one precipitation line with an extract from the brain of 8-10 week human embryo but does not react with extracts from other embryonic organs. It is also shown by immuno-electrophoresis that the glioblastoma-associated antigen has a beta-globulin mobility which distinguishes it from the embryonic brain antigen.
用人胶质母细胞瘤的兔抗血清对正常成人器官和脑肿瘤的提取物进行检测。通过琼脂糖凝胶免疫扩散法确定，该抗血清除了与胶质母细胞瘤提取物发生反应外，还与人肾、肺、肝、脾、睾丸和正常脑的提取物发生反应。在用冻干的混合人血浆以及随后用正常器官（包括正常脑）的提取物完全吸收后，它仅继续与胶质母细胞瘤提取物发生反应。这种完全吸收的抗胶质母细胞瘤抗血清与8 - 10周龄人类胚胎脑的提取物产生一条沉淀线，但不与其他胚胎器官的提取物发生反应。免疫电泳也表明，胶质母细胞瘤相关抗原具有β -球蛋白迁移率，这使其与胚胎脑抗原相区别。
