Institute for Surface Chemistry, Box 5607, S-114 86 Stockholm.
Biotechnol Bioeng. 1990 Dec 20;36(11):1083-9. doi: 10.1002/bit.260361102.
The protein releases, the particle size distribution and the viscosity of disrupted E. coli suspensions from Dyno Mill KDL, Manton Gaulin 15 M-8TA and Microfluidizer M-110 were determined. The effects of these parameters on separation of the cell debris from the protein solution by centrifugation and by filtration were also examined. All three disintegration methods investigated give approximately the same protein and enzyme releases but considerably different physical properties of the cell disintegrates which influences centrifugation and filtration. The separation degree of biomass during centrifugation is only slightly affected by increasing degree of disruption (increasing protein releases) in the bead mill, while an increase in the degree of disruption in the two high pressure homogenizers drastically reduces the centrifugal degree of separation. However, increasing degrees of disruption result in shorter filtration times during filtration for all three disintegration methods. The results show further that the cell concentration only has a minor influence on protein releases in the Microfluidizer high-pressure homogenizer, while an increase in the biomass content reduces the separability of the cell disintegrate both in filtration and in centrifugation.
测定了 Dyno Mill KDL、Manton Gaulin 15 M-8TA 和 Microfluidizer M-110 对大肠杆菌悬浮液的破坏后蛋白质释放、颗粒尺寸分布和粘度。还研究了这些参数对通过离心和过滤从蛋白质溶液中分离细胞碎片的影响。研究的所有三种破碎方法都给出了大致相同的蛋白质和酶释放，但细胞破碎的物理性质却大不相同，这会影响离心和过滤。在珠磨机中，通过增加破碎程度（增加蛋白质释放）对离心过程中生物质的分离程度的影响很小，而在两种高压均化器中增加破碎程度会大大降低离心分离程度。然而，对于所有三种破碎方法，增加破碎程度都会导致过滤时间缩短。结果还表明，在 Microfluidizer 高压均化器中，细胞浓度对蛋白质释放的影响较小，而生物质含量的增加会降低细胞破碎在过滤和离心过程中的分离性。
