Holtzapple M, Cognata M, Shu Y, Hendrickson C
Department of Chemical Engineering, Texas A&M University, College Station, Texas 77843, USA.
Biotechnol Bioeng. 1990 Jul;36(3):275-87. doi: 10.1002/bit.260360310.
Inhibition of Trichoderma reesei cellulase by sugars (glucose, delta-gluconolactone, and cellobiose) and solvents (ethanol, butanol, and acetone) was studied using cellulose azure. Glucose, cellobiose, ethanol, and butanol were noncompetitive inhibitors, delta-gluconolactone was a mixed inhibitor, and acetone was a noncompetitive activator. Converting cellobiose to glucose reduces the effective inhibitor binding constant by 6 times and converting cellobiose to ethanol reduces it by 16 times.
使用纤维素天青研究了糖（葡萄糖、δ-葡萄糖酸内酯和纤维二糖）和溶剂（乙醇、丁醇和丙酮）对里氏木霉纤维素酶的抑制作用。葡萄糖、纤维二糖、乙醇和丁醇是非竞争性抑制剂，δ-葡萄糖酸内酯是混合型抑制剂，丙酮是非竞争性激活剂。将纤维二糖转化为葡萄糖可使有效抑制剂结合常数降低6倍，将纤维二糖转化为乙醇可使其降低16倍。
