Mekala Naveen Kumar, Singhania Reeta Rani, Sukumaran Rajeev K, Pandey Ashok
Department of Biotechnology, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum, India.
Appl Biochem Biotechnol. 2008 Dec;151(2-3):122-31. doi: 10.1007/s12010-008-8156-9. Epub 2008 Feb 20.
Sugar cane bagasse was used as substrate for cellulase production using Trichoderma reesei RUT C30, and the culture parameters were optimized for enhancing cellulase yield. The culture parameters, such as incubation temperature, duration of incubation, and inducer concentration, were optimized for enhancing cellulase yield using a Box-Behnken experimental design. The optimal level of each parameter for maximum cellulase production by the fungus was determined. Predicted results showed that cellulase production was highest (25.6 FPAase units per gram dry substrate) when the inducer concentration was 0.331 ml/gds, and the incubation temperature and time were 33 degrees C and 67 h, respectively. Crude inducer generated by cellulase action was found to be very effective in inducing cellulases. Validation of predicted results was done, and the experimental values correlated well with that of the predicted.
甘蔗渣被用作里氏木霉RUT C30生产纤维素酶的底物，并对培养参数进行了优化以提高纤维素酶产量。采用Box-Behnken实验设计对培养参数（如培养温度、培养时间和诱导剂浓度）进行优化以提高纤维素酶产量。确定了该真菌产生最大量纤维素酶时每个参数的最佳水平。预测结果表明，当诱导剂浓度为0.331 ml/gds，培养温度和时间分别为33℃和67 h时，纤维素酶产量最高（每克干底物25.6 FPAase单位）。发现由纤维素酶作用产生的粗诱导剂在诱导纤维素酶方面非常有效。对预测结果进行了验证，实验值与预测值相关性良好。
