Kim D J, Chang H N
Department of Chemical Engineering, Korea Advanced Institute of Science and Technology, PO Box 131, Dongdaemun, Seoul, Korea.
Biotechnol Bioeng. 1990 Aug 20;36(5):460-6. doi: 10.1002/bit.260360505.
Plant cell cultures of Lithospermum erythrorhizon were carried out to produce shikonin by in situ extraction and cell immobilization in calcium alginate bead in shake flask cultures. In situ product extraction and cell immobilization enhanced shikonin production and facilitated product recovery. In situ extraction by n-hexadecane and cell immobilization by calcium alginate gave higher specific shikonin productivities of 7.4 and 2.5 times, respectively, than those from the cultures of free cells without extraction. Simultaneous use of both techniques increased specific and volumetric productivities of shikonin 25- and 15-fold, respectively. In calcium alginate immobilized cell cultures, n-hexadecane addition at an early stage (before 15 days) was effective for shikonin production, and solvent addition after 15 days of the culture significantly reduced shikonin production. Higher numbers of plant cell immobilized bead inoculation did not increase shikonin production and sucrose consumption. Most of the produced shikonin was dissolved in the solvent layer.
开展了紫草的植物细胞培养，以通过原位提取和在摇瓶培养中固定于海藻酸钙珠粒中的细胞来生产紫草素。原位产物提取和细胞固定化提高了紫草素的产量，并便于产物回收。与未进行提取的游离细胞培养相比，用正十六烷进行原位提取和用海藻酸钙进行细胞固定化分别使紫草素的比生产率提高了7.4倍和2.5倍。同时使用这两种技术分别使紫草素的比生产率和体积生产率提高了25倍和15倍。在海藻酸钙固定化细胞培养中，早期（15天之前）添加正十六烷对紫草素生产有效，而培养15天后添加溶剂会显著降低紫草素产量。接种更多数量的植物细胞固定化珠粒并未提高紫草素产量和蔗糖消耗量。所产生的紫草素大部分溶解于溶剂层中。
