用于检测产生黄曲霉毒素的霉菌的硅胶培养基。
Silica gel medium to detect molds that produce aflatoxin.
作者信息
Torrey G S, Marth E H
出版信息
Appl Environ Microbiol. 1976 Sep;32(3):376-80. doi: 10.1128/aem.32.3.376-380.1976.
Abstract
A chemically defined nutrient solution containing tartaric acid was made solid by mixing it with an alkaline silicate solution. Production of aflatoxin by asperfilli growing on the silica gel medium coincided with the presence of a blue fluorescent area surrounding colonies, as observed under long-wave ultraviolet light. Presence of aflatoxin in the medium was confirmed by drying the gels, extracting them with chloroform-methanol, and examining extracts for fluorescent materials by viewing them on thin-layer chromatograms under ultraviolet light.
摘要
一种含有酒石酸的化学成分明确的营养液通过与碱性硅酸盐溶液混合而固化。在硅胶培养基上生长的曲霉产生黄曲霉毒素的情况与在长波紫外线下观察到的菌落周围出现蓝色荧光区域相一致。通过干燥凝胶、用氯仿 - 甲醇提取并在紫外线下观察薄层色谱图上的提取物中的荧光物质，证实了培养基中存在黄曲霉毒素。
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