Arnett Diana R, Jennings Jennifer L, Tabb David L, Link Andrew J, Weil P Anthony
Department of Molecular Physiology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0615, USA.
Mol Cell Proteomics. 2008 Nov;7(11):2090-106. doi: 10.1074/mcp.M800221-MCP200. Epub 2008 Jul 2.
Yeast Mot1p, a member of the Snf2 ATPase family of proteins, is a transcriptional regulator that has the unusual ability to both repress and activate mRNA gene transcription. To identify interactions with other proteins that may assist Mot1p in its regulatory processes, Mot1p was purified from replicate yeast cell extracts, and Mot1p-associated proteins were identified by coupled multidimensional liquid chromatography and tandem mass spectrometry. Using this approach we generated a catalog of Mot1p-interacting proteins. Mot1p interacts with a range of transcriptional co-regulators as well as proteins involved in chromatin remodeling. We propose that interaction with such a wide range of proteins may be one mechanism through which Mot1p subserves its roles as a transcriptional activator and repressor.
酵母Mot1p是Snf2 ATP酶蛋白家族的成员，是一种转录调节因子，具有抑制和激活mRNA基因转录的非凡能力。为了鉴定与其他可能协助Mot1p进行调节过程的蛋白质的相互作用，从复制的酵母细胞提取物中纯化Mot1p，并通过多维液相色谱和串联质谱联用鉴定与Mot1p相关的蛋白质。使用这种方法，我们生成了一份Mot1p相互作用蛋白的目录。Mot1p与一系列转录共调节因子以及参与染色质重塑的蛋白质相互作用。我们提出，与如此广泛的蛋白质相互作用可能是Mot1p发挥转录激活剂和抑制剂作用的一种机制。
