Maeda Hatsuo
Hyogo University of Health Sciences, Department of Pharmacy, Division of Bioanalytical Chemistry, Kobe, Japan.
Ann N Y Acad Sci. 2008;1130:149-56. doi: 10.1196/annals.1430.012.
The fluorometric measurement of an individual reactive oxygen species (ROS) provides useful biological and biochemical information on ROS as important mediators for the pathological conditions of various diseases and yet requires a highly specific probe toward the target ROS. To design such a specific ROS probe, this report proposes a new strategy based on protection-deprotection chemistry between fluoresceins and their derivatives protected with benzenesulfonyl groups. The strategy has allowed developing new fluorescent probes toward extra- and intracellular hydrogen peroxide or superoxide. Herein, I outline the strategy used for the design of these ROS probes and their probe performance with high selectivity toward hydrogen peroxide and superoxide, which could not be achieved until a simple deprotection, namely, a nonredox reaction, was used as a fluorescing process.
对单个活性氧（ROS）进行荧光测量可为ROS提供有用的生物学和生物化学信息，ROS是多种疾病病理状况的重要介质，但这需要针对目标ROS的高度特异性探针。为了设计这样一种特异性ROS探针，本报告提出了一种基于荧光素及其苯磺酰基保护衍生物之间的保护-去保护化学的新策略。该策略已使针对细胞外和细胞内过氧化氢或超氧阴离子的新型荧光探针得以开发。在此，我概述了用于设计这些ROS探针的策略及其对过氧化氢和超氧阴离子具有高选择性的探针性能，直到将简单的去保护反应（即非氧化还原反应）用作荧光过程，才实现了这种高选择性。
