Sánchez-Moreiras Adela M, de la Peña Teodoro Coba, Reigosa Manuel J
Department of Plant Biology and Soil Science, University of Vigo, Campus Lagoas-Marcosende s/n, E-36310 Vigo, Spain.
Phytochemistry. 2008 Aug;69(11):2172-9. doi: 10.1016/j.phytochem.2008.05.014. Epub 2008 Jul 1.
Benzoxazolin-2(3H)-one (BOA) is a natural plant product that is phytotoxic to target plant species, inhibiting germination and growth and causing oxidative damage. We investigated its effects on the root meristems of seedlings of lettuce (Lactuca sativa) by means of light and transmission electron microscopy, flow cytometry, and conventional determination of mitotic index. Flow cytometry analyses and mitotic index showed a retard of cell cycle in BOA-treated meristems with selective activity at G2/M checkpoint.
苯并恶唑啉-2(3H)-酮(BOA)是一种天然植物产物,对目标植物物种具有植物毒性,会抑制种子萌发和生长并造成氧化损伤。我们通过光学显微镜、透射电子显微镜、流式细胞术以及常规的有丝分裂指数测定,研究了其对生菜(Lactuca sativa)幼苗根分生组织的影响。流式细胞术分析和有丝分裂指数显示,经BOA处理的分生组织细胞周期延迟,在G2/M期检查点具有选择性活性。