Coster Martin E, Stiles Jean, Krohne Sheryl G, Raskin Rose E
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
J Am Vet Med Assoc. 2008 Jun 15;232(12):1825-33. doi: 10.2460/javma.232.12.1825.
OBJECTIVE-To report values for tear production, central corneal touch threshold (CTT), and intraocular pressure (IOP) in healthy guinea pigs and determine results of aerobic bacterial culture and cytologic examination of conjunctival swab specimens. DESIGN-Cross-sectional study. ANIMALS-31 Healthy guinea pigs (62 eyes) of various ages and breeds. PROCEDURES-Tear production was measured by the phenol red thread tear test (PRT) and Schirmer tear test (STT) before and after topical anesthetic application, CTT was measured with an esthesiometer, and IOP was measured by applanation tonometry. RESULTS-Combining data from all eyes, mean +/- SD PRT values before and after topical anesthetic administration were 21.26 +/- 4.19 mm/15 s and 22.47 +/- 3.31 mm/15 s, respectively, and mean IOP was 18.27 +/- 4.55 mm Hg. Median STT values before and after topical anesthetic administration were 3 mm/min (range, 0 to 12 mm/min) and 4 mm/min (range, 0 to 11 mm/min), respectively, and median CTT was 2.0 cm (range, 0.5 to 3.0 cm). Values did not differ between eyes for any test, but significant differences were identified for PRT values between males and females and between values obtained before and after topical anesthetic administration. Common bacterial isolates included Corynebacterium spp, Streptococcus spp, and Staphylococcus spp. Cytologic examination of conjunctival swab specimens revealed mainly basal epithelial cells; lymphocytes were common. CONCLUSIONS AND CLINICAL RELEVANCE-Results provided information on values for PRT, STT, CTT, and IOP in healthy guinea pigs and on expected findings for aerobic bacterial culture and cytologic examination of conjunctival swab specimens.
目的——报告健康豚鼠的泪液分泌量、中央角膜触觉阈值（CTT）和眼压（IOP）值，并确定结膜拭子标本的需氧细菌培养和细胞学检查结果。设计——横断面研究。动物——31只不同年龄和品种的健康豚鼠（62只眼）。方法——在局部麻醉应用前后，通过酚红棉线泪液试验（PRT）和Schirmer泪液试验（STT）测量泪液分泌量，用感觉计测量CTT，用压平眼压计测量IOP。结果——综合所有眼睛的数据，局部麻醉给药前后的平均±标准差PRT值分别为21.26±4.19mm/15秒和22.47±3.31mm/15秒，平均IOP为18.27±4.55mmHg。局部麻醉给药前后的STT中位数分别为3mm/分钟（范围为0至12mm/分钟）和4mm/分钟（范围为0至11mm/分钟），CTT中位数为2.0cm（范围为0.5至3.0cm）。任何一项测试中两眼之间的值均无差异，但在雄性和雌性之间以及局部麻醉给药前后获得的值之间，PRT值存在显著差异。常见的细菌分离株包括棒状杆菌属、链球菌属和葡萄球菌属。结膜拭子标本的细胞学检查主要显示基底上皮细胞；淋巴细胞很常见。结论及临床意义——结果提供了健康豚鼠的PRT、STT、CTT和IOP值以及结膜拭子标本需氧细菌培养和细胞学检查的预期结果的信息。
