de Roos Baukje, McArdle Harry J
Division of Vascular Health, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, United Kingdom.
Br J Nutr. 2008 Jun;99 Suppl 3:S66-71. doi: 10.1017/S0007114508006909.
Nutrition research has slowly started to adopt the proteomics techniques to measure changes in the protein complement of a biological system. This enables modelling of biological processes in response to dietary interventions, as well as the elucidation of novel biomarkers for health or disease that are sensitive to such interventions. There are limited studies on the effect of micronutrients on the proteome, so this review concentrates rather more on dietary intervention studies that have used proteomics (mainly classical 2D gel electrophoresis combined with mass spectrometry) to elucidate changes in pathways that relate to glucose and fatty acid metabolism, oxidative stress, anti-oxidant defence mechanisms and redox status. The ability to measure regulation of more low abundant proteins, such as those involved in inflammatory pathways, as well as the evaluation and validation of newly discovered candidate biomarkers in human biofluids, may depend on the introduction of more quantitative and sensitive methods like multiple reaction monitoring (MRM) and multiplexed immunoassays in nutrition research.
营养研究已逐渐开始采用蛋白质组学技术来测定生物系统中蛋白质组成的变化。这有助于对饮食干预所引起的生物过程进行建模，还能阐明对这类干预敏感的健康或疾病新生物标志物。关于微量营养素对蛋白质组影响的研究有限，因此本综述更多地聚焦于饮食干预研究，这些研究运用蛋白质组学（主要是经典的二维凝胶电泳结合质谱法）来阐明与葡萄糖和脂肪酸代谢、氧化应激、抗氧化防御机制及氧化还原状态相关途径的变化。测量更多低丰度蛋白质（如参与炎症途径的蛋白质）调控的能力，以及在人体生物流体中对新发现的候选生物标志物进行评估和验证，可能取决于营养研究中引入更定量、更灵敏的方法，如多反应监测（MRM）和多重免疫测定法。
