Feng Kun, Wang Lan-Ying, Liao Dong-Jiang, Lu Xin-Peng, Hu De-Jun, Liang Xiao, Zhao Jing, Mo Zi-Yao, Li Shao-Ping
State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macao, China.
Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, China.
Mycology. 2017 Aug 30;8(4):231-258. doi: 10.1080/21501203.2017.1365314. eCollection 2017.
The fruiting body formation mechanisms of are still unclear. To explore the mechanisms, proteins potentially related to the fruiting body formation, proteins from fruiting bodies, and mycelia of species were assessed by using two-dimensional fluorescence difference gel electrophoresis, and the differential expression proteins were identified by matrix-assisted laser desorption/ionisation tandem time of flight mass spectrometry. The results showed that 198 differential expression proteins (252 protein spots) were identified during the fruiting body formation of species, and 24 of them involved in fruiting body development in both and other microorganisms. Especially, enolase and malate dehydrogenase were first found to play an important role in fruiting body development in macro-fungus. The results implied that cAMP signal pathway involved in fruiting body development of , meanwhile glycometabolism, protein metabolism, energy metabolism, and cell reconstruction were more active during fruiting body development. It has become evident that fruiting body formation of is a highly complex differentiation process and requires precise integration of a number of fundamental biological processes. Although the fruiting body formation mechanisms for all these activities remain to be further elucidated, the possible mechanism provides insights into the culture of .
[具体物种]的子实体形成机制仍不清楚。为了探究这些机制，通过二维荧光差异凝胶电泳对[具体物种]子实体形成过程中潜在相关的蛋白质、子实体及菌丝体中的蛋白质进行了评估，并采用基质辅助激光解吸/电离串联飞行时间质谱法对差异表达蛋白质进行了鉴定。结果表明，在[具体物种]子实体形成过程中鉴定出198种差异表达蛋白质（252个蛋白点），其中24种在[具体物种]及其他微生物的子实体发育中均有涉及。特别是，首次发现烯醇化酶和苹果酸脱氢酶在大型真菌子实体发育中起重要作用。结果表明，cAMP信号通路参与了[具体物种]的子实体发育，同时糖代谢、蛋白质代谢、能量代谢和细胞重建在子实体发育过程中更为活跃。显然，[具体物种]的子实体形成是一个高度复杂的分化过程，并需要许多基本生物学过程的精确整合。尽管所有这些活动的子实体形成机制仍有待进一步阐明，但所提出的可能机制为[具体物种]的培养提供了思路。
