Qiu Wei-Gang, Bruno John F, McCaig William D, Xu Yun, Livey Ian, Schriefer Martin E, Luft Benjamin J
Hunter College of the City University of New York, New York, New York, USA.
Emerg Infect Dis. 2008 Jul;14(7):1097-104. doi: 10.3201/eid1407.070880.
The A and B clones of Borrelia burgdorferi sensu stricto, distinguished by outer surface protein C (ospC) gene sequences, are commonly associated with disseminated Lyme disease. To resolve phylogenetic relationships among isolates, we sequenced 68 isolates from Europe and North America at 1 chromosomal locus (16S-23S ribosomal RNA spacer) and 3 plasmid loci (ospC,dbpA, and BBD14). The ospC-A clone appeared to be highly prevalent on both continents, and isolates of this clone were uniform in DNA sequences, which suggests a recent trans-oceanic migration. The genetic homogeneity of ospC-A isolates was confirmed by sequences at 6 additional chromosomal housekeeping loci (gap, alr, glpA, xylB, ackA, and tgt). In contrast, the ospC-B group consists of genotypes distinct to each continent, indicating geographic isolation. We conclude that the ospC-A clone has dispersed rapidly and widely in the recent past. The spread of the ospC-A clone may have contributed, and likely continues to contribute, to the rise of Lyme disease incidence.
狭义伯氏疏螺旋体的A和B克隆，可通过外表面蛋白C（ospC）基因序列区分，通常与播散性莱姆病相关。为解析分离株之间的系统发育关系，我们对来自欧洲和北美的68株分离株的1个染色体位点（16S - 23S核糖体RNA间隔区）和3个质粒位点（ospC、dbpA和BBD14）进行了测序。ospC - A克隆似乎在两大洲都高度流行，且该克隆的分离株DNA序列一致，这表明近期发生了跨洋迁移。ospC - A分离株的遗传同质性通过另外6个染色体管家基因位点（gap、alr、glpA、xylB、ackA和tgt）的序列得到证实。相比之下，ospC - B组由每个大陆特有的基因型组成，表明存在地理隔离。我们得出结论，ospC - A克隆在近期已迅速广泛传播。ospC - A克隆的传播可能已经并可能继续促使莱姆病发病率上升。
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