Saleh Mostafa A
Biochemistry Unit, Regional Animal Health Research Laboratory, Animal Health Research Institute, EL-Kharga, El-Wadi El-Gadid 725211, Egypt.
Res Vet Sci. 2009 Feb;86(1):43-8. doi: 10.1016/j.rvsc.2008.05.005. Epub 2008 Jul 2.
This study aimed to determine the erythrocytic lipid peroxidation and haemoglobin oxidation as contributory factors causing anaemia in cattle (Friesian x Egyptian native breed) infected with Babesia bigemina. Blood was collected from 32 cows infected with B. bigemina along with 18 healthy cows as controls for determination of erythrocytic malondialdehyde (MDA), blood methaemoglobin (MetHb), plasma free haemoglobin (PHb), corpuscular osmotic fragility (COF), red blood cell count (RBC), total haemoglobin (Hb) and packed cell volume (PCV). Percentage of parasitaemia varied from 14% to 36%. MDA, MetHb, COF and PHb were significantly increased (P<0.001) in infected cows versus controls. Parasitaemia was positively correlated (P<0.001) with MDA, MetHb, COF and PHb. MDA was positively correlated (P<0.001) with COF and PHb and negatively correlated (P<0.001) with RBC, Hb and PCV. MetHb was negatively correlated (P<0.001) with RBC, Hb and PCV and positively correlated (P<0.001) with COF. In conclusion, B. bigemina infection in cattle is associated with a parasitic burden-dependent corpuscular oxidative damage as indicated by membrane lipid peroxidation and methaemoglobin formation, which are contributed to COF and intravascular haemolysis.
本研究旨在确定红细胞脂质过氧化和血红蛋白氧化作为导致感染双芽巴贝斯虫的牛（黑白花×埃及本地品种）贫血的促成因素。从32头感染双芽巴贝斯虫的奶牛以及18头健康奶牛采集血液，用于测定红细胞丙二醛（MDA）、血液高铁血红蛋白（MetHb）、血浆游离血红蛋白（PHb）、红细胞渗透脆性（COF）、红细胞计数（RBC）、总血红蛋白（Hb）和血细胞比容（PCV）。寄生虫血症百分比在14%至36%之间。与对照组相比，感染奶牛的MDA、MetHb、COF和PHb显著升高（P<0.001）。寄生虫血症与MDA、MetHb、COF和PHb呈正相关（P<0.001）。MDA与COF和PHb呈正相关（P<0.001），与RBC、Hb和PCV呈负相关（P<0.001）。MetHb与RBC、Hb和PCV呈负相关（P<0.001），与COF呈正相关（P<0.001）。总之，牛感染双芽巴贝斯虫与寄生虫负荷依赖性的红细胞氧化损伤有关，如膜脂质过氧化和高铁血红蛋白形成所示，这导致了COF和血管内溶血。
