Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, P.O. Box 1177, Urmia, Iran.
Vet Parasitol. 2012 Apr 30;185(2-4):124-30. doi: 10.1016/j.vetpar.2011.10.001. Epub 2011 Oct 8.
The present study aimed to assess antioxidant status and oxidative stress in sheep naturally infected with Babesia ovis. Red blood cell (RBC) count, hemoglobin (Hb) concentration, packed cell volume (PCV), piroplasm parasitemia percentage, malondialdehyde (MDA) concentration, erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities and total antioxidant capacity (TAC) were determined in 52 sheep naturally infected with B. ovis as well as same number of healthy sheep in West-Azerbaijan province, Iran. Microscopic examination of Giemsa-stained peripheral blood smears revealed B. ovis infection. The parasitological diagnosis was confirmed using polymerase chain reaction (PCR) analysis by amplifying a partial small subunit ribosomal RNA (ssu rRNA) gene sequence of B. ovis of 52 diseased sheep, 18 (34.61%), 11 (21.15%), 16 (30.76%) and 7 (13.48%) had <1%, 1-2%, 2-3% and >3% parasitemia, respectively. Compared to controls, the activities of erythrocyte GSH-Px, SOD, TAC and CAT showed a significant decrease, whereas the concentration of MDA in erythrocytes of infected sheep increased significantly. Parasitemia rate was positively correlated with MDA and negatively correlated with PCV, SOD, CAT, GSH-Px and TAC. Also, MDA was negatively correlated with PCV, SOD, catalase, GSH-Px and TAC. The study demonstrated that B. ovis plays an important role in the occurrence of oxidative damage to RBCs and anemia in ovine babesiosis.
本研究旨在评估天然感染巴贝西亚虫的绵羊的抗氧化状态和氧化应激。在伊朗东阿塞拜疆省，我们测定了 52 只天然感染 B. ovis 的绵羊以及相同数量的健康绵羊的红细胞（RBC）计数、血红蛋白（Hb）浓度、红细胞压积（PCV）、焦虫血症寄生虫百分比、丙二醛（MDA）浓度、红细胞超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）活性和总抗氧化能力（TAC）。吉姆萨染色外周血涂片的显微镜检查显示 B. ovis 感染。通过扩增 52 只患病绵羊的部分小亚基核糖体 RNA（ssu rRNA）基因序列的聚合酶链反应（PCR）分析，对寄生虫病进行了诊断。结果发现，18（34.61%）、11（21.15%）、16（30.76%）和 7（13.48%）只患病绵羊的寄生虫血症率分别为<1%、1-2%、2-3%和>3%。与对照组相比，红细胞 GSH-Px、SOD、TAC 和 CAT 的活性显著降低，而感染绵羊红细胞中的 MDA 浓度显著升高。寄生虫血症率与 MDA 呈正相关，与 PCV、SOD、CAT、GSH-Px 和 TAC 呈负相关。此外，MDA 与 PCV、SOD、CAT、GSH-Px 和 TAC 呈负相关。该研究表明，B. ovis 在绵羊巴贝斯虫病中红细胞氧化损伤和贫血的发生中起着重要作用。
