Ozcan Levent, Sahin Yücel, Türk Hayrettin
Faculty of Science, Department of Chemistry, Anadolu University, Yunus Emre Campus, Tepebasi, 26470 Eskişehir, Turkey.
Biosens Bioelectron. 2008 Dec 1;24(4):512-7. doi: 10.1016/j.bios.2008.05.004. Epub 2008 May 27.
An enzymeless biosensor, based on electrodeposition of overoxidized polypyrrole nanofiber onto pencil graphite electrode and modified with cobalt(II) phthalocyanine tetrasulfonate (CoPcTS), was investigated in this study. CoPcTS showed electrocatalytic activity for the oxidation of glucose in alkaline solution. The electrochemical performance of the modified electrodes was investigated by differential pulse voltammetric (DPV) method. The resulting biosensor exhibited excellent performance for glucose determination with a wide linear range (0.25-20mM), a highly reproducible response (R.S.D. of 2.7%), low percentage of the interferences and long-term stability. The calculated detection limit was 0.1mM at 3sigma. In order to verify the reliability of the biosensor, it was applied to the determination of glucose in serum samples. The results were satisfactory and agreed closely with those measured in a hospital.
本研究考察了一种无酶生物传感器，该传感器基于在铅笔石墨电极上电沉积过氧化聚吡咯纳米纤维并以四磺酸钴酞菁（CoPcTS）进行修饰。CoPcTS在碱性溶液中对葡萄糖氧化表现出电催化活性。采用差分脉冲伏安法（DPV）研究了修饰电极的电化学性能。所得生物传感器在葡萄糖测定方面表现出优异性能，线性范围宽（0.25 - 20 mM），响应具有高度重现性（相对标准偏差为2.7%），干扰百分率低且具有长期稳定性。在3σ时计算得到的检测限为0.1 mM。为验证该生物传感器的可靠性，将其应用于血清样品中葡萄糖的测定。结果令人满意，与医院测量值密切相符。
