Arun A B, Schumann Peter, Chu Horng-I, Tan Chen-Chung, Chen Wen-Ming, Lai Wei-An, Kämpfer Peter, Shen Fo-Ting, Rekha P D, Hung Mei-Hua, Chou Jui-Hsing, Young Chiu-Chung
College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Int J Syst Evol Microbiol. 2008 Jul;58(Pt 7):1571-5. doi: 10.1099/ijs.0.65206-0.
A Gram-type negative or variable, aerobic, rod-shaped, nitrogen-fixing bacterium, designated strain CC4(T), was isolated on nutrient agar from a soil sample collected at a regional agricultural research field station located in Kaohsiung County, Taiwan. 16S rRNA gene sequence analysis demonstrated that this isolate is unique, showing <92.5 % similarity with respect to species of the genera Xanthobacter (maximum of 92.2 % similarity), Prosthecomicrobium (92.0 %), Devosia (91.9 %), Kaistia (91.9 %) and Methylocystis (91.9 %). The organism utilized acetic acid, formic acid, beta-hydroxybutyric acid, dl-lactic acid, succinic acid, bromosuccinic acid, l-alaninamide, l-alanine, l-alanyl glycine, l-glutamic acid and l-proline as substrates, but not methanol or methylamine. Chemotaxonomic data revealed that strain CC4(T) contains ubiquinone Q-10 as the major respiratory quinone. The characteristic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, an unknown lipid and an unknown aminolipid. The fatty acid profile differed from those of members of the genera Xanthobacter, Prosthecomicrobium, Devosia and Kaistia. The predominant fatty acids were C(18 : 1)omega7c, C(19 : 0) cyclo and C(16 : 0). On the basis of 16S rRNA gene sequence analysis in combination with chemotaxonomic and physiological data, strain CC4(T) represents a novel genus and species, for which we propose the name Pseudoxanthobacter soli gen. nov., sp. nov. The type strain of Pseudoxanthobacter soli is CC4(T) (=DSM 19599(T) =CIP 109513(T)).
从台湾高雄县一个区域农业研究田间站采集的土壤样本中，在营养琼脂上分离出一株革兰氏阴性或可变、需氧、杆状、固氮细菌，命名为菌株CC4(T)。16S rRNA基因序列分析表明，该分离株具有独特性，与黄杆菌属、突柄微菌属、德沃斯氏菌属、凯斯氏菌属和甲基孢囊菌属的物种相似度均低于92.5%（最高相似度为92.2%）。该菌株利用乙酸、甲酸、β-羟基丁酸、dl-乳酸、琥珀酸、溴琥珀酸、l-丙氨酰胺、l-丙氨酸、l-丙氨酰甘氨酸、l-谷氨酸和l-脯氨酸作为底物，但不利用甲醇或甲胺。化学分类学数据显示，菌株CC4(T)含有泛醌Q-10作为主要呼吸醌。肽聚糖的特征二氨基酸是内消旋二氨基庚二酸。主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、磷脂酰单甲基乙醇胺、磷脂酰二甲基乙醇胺、一种未知脂类和一种未知氨基脂类。脂肪酸谱与黄杆菌属、突柄微菌属、德沃斯氏菌属和凯斯氏菌属成员不同。主要脂肪酸为C(18 : 1)ω7c、C(19 : 0)环丙烷和C(16 : 0)。基于16S rRNA基因序列分析并结合化学分类学和生理学数据，菌株CC4(T)代表一个新的属和种，我们将其命名为土壤假黄杆菌属新属，新种。土壤假黄杆菌的模式菌株为CC4(T)（=DSM 19599(T)=CIP 109513(T)）。
