Hwang Chung Y, Cho Byung C
School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Jul;58(Pt 7):1591-7. doi: 10.1099/ijs.0.65587-0.
A Gram-negative, rod-shaped, strictly aerobic micro-organism, designated strain CL-GR60(T), was isolated from coastal seawater from the East Sea, Korea. A 16S rRNA gene sequence analysis revealed a clear affiliation with the family Hyphomicrobiaceae. Phylogenetic analyses showed that strain CL-GR60(T) formed a robust cluster with the species of the genus Devosia at sequence similarity levels of 91.1-93.1 %; no other species in the family Hyphomicrobiaceae shared more than 90 % sequence similarity with strain CL-GR60(T). The strain grew optimally in the presence of 3-4 % sea salts at 30-35 degrees C and pH 7. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, two unidentified glycolipids, an unidentified phospholipid and an unidentified lipid. The strain contained C(18 : 1)omega7c (54.7 %), 11-methyl C(18 : 1)omega7c (19.3 %), C(18 : 0) (14.4 %) as the predominant fatty acids. Ubiquinone-10 was the major quinone. The DNA G+C content was 62.9 mol%. On the basis of physiological data, fatty acid composition and results of phylogenetic analysis of the 16S rRNA gene sequence, strain CL-GR60(T) represents a novel genus and species within the family Hyphomicrobiaceae, for which the name Cucumibacter marinus gen. nov., sp. nov. is proposed. The type strain of Cucumibacter marinus is CL-GR60(T) (=KCCM 90027(T) =DSM 18995(T)).
从韩国东海的沿海海水中分离出一株革兰氏阴性、杆状、严格需氧的微生物，命名为CL-GR60(T)菌株。16S rRNA基因序列分析表明，它与生丝微菌科有明确的亲缘关系。系统发育分析表明，CL-GR60(T)菌株与德沃斯氏菌属的物种在序列相似性水平为91.1-93.1%时形成了一个稳健的聚类；生丝微菌科中没有其他物种与CL-GR60(T)菌株的序列相似性超过90%。该菌株在30-35℃、pH 7以及3-4%海盐存在的条件下生长最佳。主要极性脂类为磷脂酰甘油、双磷脂酰甘油、两种未鉴定的糖脂、一种未鉴定的磷脂和一种未鉴定的脂类。该菌株以C(18 : 1)ω7c（54.7%）、11-甲基C(18 : 1)ω7c（19.3%）、C(18 : 0)（14.4%）作为主要脂肪酸。泛醌-10是主要的醌类。DNA G+C含量为62.9 mol%。基于生理数据、脂肪酸组成以及16S rRNA基因序列的系统发育分析结果，CL-GR60(T)菌株代表了生丝微菌科内的一个新属和新种，为此提出新属名黄瓜杆菌属（Cucumibacter），新种名海洋黄瓜杆菌（Cucumibacter marinus）。海洋黄瓜杆菌的模式菌株为CL-GR60(T)（=KCCM 90027(T) =DSM 18995(T)）。
