Moskovtsev Sergey I, Librach Clifford L
CReATe Fertility Center, Toronto, ON, Canada.
Methods Mol Biol. 2013;927:13-9. doi: 10.1007/978-1-62703-038-0_2.
Sperm vitality is a reflection of the proportion of live, membrane-intact spermatozoa determined by either dye exclusion or osmoregulatory capacity under hypo-osmotic conditions. In this chapter we address the two most common methods of sperm vitality assessment: eosin-nigrosin staining and the hypo-osmotic swelling test, both utilized in clinical Andrology laboratories.
