Lee Hae-Won, Ten Irina L, Jung Hae-Min, Liu Qing-Mei, Im Wan-Taek, Lee Sung-Taik
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea.
J Microbiol Biotechnol. 2008 Jun;18(6):1011-5.
A Gram-negative, strictly aerobic, motile bacterial strain, designated Gsoil 124T, was isolated from a soil sample taken from a ginseng field in Pocheon Province (South Korea). The isolate contained Q-10 as the predominant lipoquinone, plus C18:1 7c and summed feature 4 (C16:1 6c and/or iso- C15:0 2-OH) as the major fatty acids. The G+C content of the genomic DNA was 68.1 mol%, and the major polar lipids consisted of sphingoglycolipid, phosphatidylglycerol, phosphatidylcholine, and phosphatidylethanolamine. A comparative 16S rRNA gene sequence analysis showed that strain Gsoil 124T was most closely related to Sphingopyxis chilensis (98.7%), Sphingopyxis alaskensis (98.2%), Sphingopyxis witflariensis (98.2%), Sphingopyxis taejonensis (98.0%), and Sphingopyxis macrogoltabida (97.6%). However, the DNA-DNA relatedness between strain Gsoil 124T and its phylogenetically closest neighbors was less than 22%. Thus, on the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 124T should be classified as representing a novel species in the genus Sphingopyxis, for which the name Sphingopyxis panaciterrae sp. nov. is proposed. The type strain is Gsoil 124T (=KCTC 12580T=LMG 24003T).
从韩国抱川市人参田采集的土壤样本中分离出一株革兰氏阴性、严格需氧、具运动性的细菌菌株，命名为Gsoil 124T。该分离菌株以Q-10作为主要的脂醌，主要脂肪酸为C18:1 ω7c以及总和特征4（C16:1 ω6c和/或异-C15:0 2-OH）。基因组DNA的G+C含量为68.1 mol%，主要极性脂类包括鞘糖脂、磷脂酰甘油、磷脂酰胆碱和磷脂酰乙醇胺。比较16S rRNA基因序列分析表明，菌株Gsoil 124T与智利鞘氨醇单胞菌（98.7%）、阿拉斯加鞘氨醇单胞菌（98.2%）、维氏鞘氨醇单胞菌（98.2%）、大田鞘氨醇单胞菌（98.0%）和大谷鞘氨醇单胞菌（97.6%）关系最为密切。然而，菌株Gsoil 124T与其系统发育关系最近的近缘菌株之间的DNA-DNA相关性小于22%。因此，基于其表型特征和系统发育独特性，菌株Gsoil 124T应被归类为鞘氨醇单胞菌属中的一个新物种，为此提出新名称人参土鞘氨醇单胞菌（Sphingopyxis panaciterrae sp. nov.）。模式菌株为Gsoil 124T（=KCTC 12580T=LMG 24003T）。
