Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701, Korea.
J Microbiol Biotechnol. 2010 Jan;20(1):15-20.
A Gram negative, aerobic, nonspore-forming, straight or curved rod-shaped bacterium, designated Gsoil 317T, was isolated from soil of a ginseng field in Pocheon Province (South Korea) and was characterized using a polyphasic approach. Cells were dimorphic, with stalk (or prostheca) and nonmotile or nonstalked and motile, by means of a single polar flagellum. Comparative analysis of 16S rRNA gene sequences revealed that strain Gsoil 317T was most closely related to Caulobacter mirabilis LMG 24261T (97.2%), Caulobacter fusiformis ATCC 15257T (97.1%), Caulobacter segnis LMG 17158T (97.0%), Caulobacter vibrioides DSM 9893T (96.8%), and Caulobacter henricii ATCC 15253T (96.7%). The sequence similarities to any other recognized species within Alphaproteobacteria were less than 96.0%. The detection of Q-10 as the major respiratory quinone and a fatty acid profile with summed feature 7 (C18:1 omega7c and/or C18:1 omega9t and/or C18:1 omega12t; 56.6%) and C16:0 (15.9%) as the major fatty acids supported the affiliation of strain Gsoil 317T to the genus Caulobacter. The G+C content of the genomic DNA was 65.5 mol%. DNA-DNA hybridization experiments showed that the DNA-DNA relatedness values between strain Gsoil 317T and its closest phylogenetic neighbors were below 11%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 317T should be classified as representing a novel species in the genus Caulobacter, for which the name Caulobacter ginsengisoli sp. nov. is proposed. The type strain is Gsoil 317T (=KCTC 12788T= DSM 18695T).
一株革兰氏阴性、需氧、非孢子形成、直或弯曲的杆状细菌，命名为 Gsoil 317T，从韩国抱川人参田的土壤中分离得到，并采用多相方法进行了特征描述。细胞具有二态性，有菌柄（或菌托）和非运动或无菌柄和运动的，通过单一极鞭毛。16S rRNA 基因序列的比较分析表明，菌株 Gsoil 317T 与 Caulobacter mirabilis LMG 24261T（97.2%）、Caulobacter fusiformis ATCC 15257T（97.1%）、Caulobacter segnis LMG 17158T（97.0%）、Caulobacter vibrioides DSM 9893T（96.8%）和 Caulobacter henricii ATCC 15253T（96.7%）最为密切相关。与α变形菌中任何其他公认种的序列相似性均小于 96.0%。主要呼吸醌 Q-10 的检测以及脂肪酸图谱（特征 7，包括 C18:1 omega7c 和/或 C18:1 omega9t 和/或 C18:1 omega12t；56.6%）和 C16:0（15.9%）为主脂肪酸，支持菌株 Gsoil 317T 属于 Caulobacter 属。基因组 DNA 的 G+C 含量为 65.5mol%。DNA-DNA 杂交实验表明，菌株 Gsoil 317T 与其最接近的系统发育邻居之间的 DNA-DNA 相关值低于 11%。基于其表型特性和系统发育上的独特性，菌株 Gsoil 317T 应归类为属于 Caulobacter 属的一个新种，建议将其命名为 Caulobacter ginsengisoli sp. nov.。模式菌株为 Gsoil 317T（=KCTC 12788T=DSM 18695T）。
