Atkinson Sarah D, Moore Jonathan E, Shah Sunil, Sharma Anant, Best Richard M, Leccisotti Antonio, Alarbi Mohammad, Rimmer David, Gardiner Tom, Moore Tara C B
Centre for Molecular Biosciences, School of Biomedical Sciences, Coleraine, Northern Ireland.
Curr Eye Res. 2008 Jul;33(7):551-8. doi: 10.1080/02713680802187453.
Compare expression of p63 in pterygium and laryngo-onycho-cutaneous (LOC) syndrome with normal conjunctiva.
P63 immunohistochemical detection was carried out in normal, pterygium, and LOC conjunctival tissue. In vitro, growth of normal conjunctival biopsy specimens, pterygium, and LOC in growth tissue was compared.
In normal conjunctiva, p63 was poorly expressed in the infranasal quadrant, with 36% of cells stained vs. 55 to 59% in other quadrants (p < 0.05). In pterygium, p63 was overexpressed (59% cells stained) compared to normal supranasal (55%) and normal infranasal conjunctiva (36%, p < 0.05). In LOC, p63 was only expressed in 39% of cells vs. 58% in normal supratemporal conjunctiva (p < 0.05). Cytokeratin 19 was expressed by all cells cultured from normal conjunctival tissue.
This study emphasizes the importance of using control tissue explanted from the correspondent conjunctival quadrant when studying proliferative disorders. Different pathogenesis may account for the differences in p63 expression between pterygium and LOC.
比较翼状胬肉和喉-甲-皮肤（LOC）综合征中p63的表达与正常结膜的差异。
对正常、翼状胬肉和LOC综合征的结膜组织进行p63免疫组化检测。在体外，比较正常结膜活检标本、翼状胬肉和LOC综合征生长组织的生长情况。
在正常结膜中，p63在下鼻象限表达较弱，36%的细胞染色，而其他象限为55%至59%（p<0.05）。与正常上鼻结膜（55%）和正常下鼻结膜（36%，p<0.05）相比，翼状胬肉中p63过度表达（59%的细胞染色）。在LOC综合征中，p63仅在39%的细胞中表达，而正常颞上结膜为58%（p<0.05）。所有从正常结膜组织培养的细胞均表达细胞角蛋白19。
本研究强调在研究增殖性疾病时使用来自相应结膜象限的对照组织的重要性。不同的发病机制可能解释了翼状胬肉和LOC综合征之间p63表达的差异。
