Department of Food Science, Food and Bioprocess Engineering Group, Agricultural University, Wageningen, The Netherlands.
Biotechnol Bioeng. 1991 Jul;38(3):232-40. doi: 10.1002/bit.260380304.
A dynamic model that predicts substrate and biomass concentration profiles across gel beads and from that the overall substrate consumption rate by the gel beads containing growing cells was evaluated with immobilized Nitrobacter agilis cells in an airlift loop reactor with oxygen as the limiting substrate. The model predictions agreed well with the observed oxygen consumption rates at three different liquid phase oxygen concentrations. Image analysis showed that 90% of the immobilized cells after 42 days of cultivation was situated in the outer shells in a film of 140 mum, while the bead radius was about 1 mm. The maximum biomass concentration in the outmost film of 56 mum was 11 kg . m(-3) gel.
采用气升式环流反应器，以氧气为限制基质，利用固定化硝化菌细胞，对基质和生物量在凝胶珠内及整体的浓度分布进行了研究，并在此基础上建立了预测模型，计算了含生长细胞的凝胶珠的总体基质消耗速率。模型预测值与在三种不同液相氧浓度下观察到的耗氧速率吻合较好。图像分析表明，培养 42 天后，90%的固定化细胞位于 140μm 厚的膜的外壳层中，而珠半径约为 1mm。最外层 56μm 厚的膜中的最大生物量浓度为 11kg·m(-3)凝胶。
