Department of Ecology and Evolution, State University of New York at Stony Brook, Stony Brook, NY 11794-5245, USA.
Biotechnol Bioeng. 1992 Mar 15;39(6):589-95. doi: 10.1002/bit.260390602.
Determinations of the DeltaG(0)' accompanying the growth of Escherichia coli K-12 on succinic acid are made using 2 different methods. The DeltaG(0)' accompanying catabolism could be calculated directly because the thermodynamic properties of the reactants and products are known. The DeltaG'accompanying anabolism could not be calculated directly because the DeltaG(f) value for a unit mass of cells was not known. A description is given of a deduction that the DeltaG' accompanying anabolism is zero, or nearly so. This is followed by a description of 2 methods, whereby the free energy of formation of a unit quantity of cellular substance can be calculated. The 2 values obtained by these methods are used to calculate the free-energy change accompanying anabolism, the resultant values being 1.72 and -11.68 kJ, respectively, with an average of -4.98 kJ (-1.19 kcal). This value is sufficiently close to zero that it can be considered to be so, indicating that the DeltaG' accompanying metabolism is that of catabolism alone.
使用 2 种不同方法测定大肠杆菌 K-12 在琥珀酸上生长时的 ΔG°'。因为反应物和产物的热力学性质是已知的，所以可以直接计算伴随分解代谢的 ΔG°'。因为不知道单位质量细胞的 ΔG°f 值，所以不能直接计算伴随合成代谢的 ΔG°'。描述了一种推断，即伴随合成代谢的 ΔG°'为零或几乎为零。接下来描述了 2 种方法，可以通过这些方法计算单位细胞物质形成的自由能。用这两种方法得到的值来计算伴随合成代谢的自由能变化，结果分别为 1.72 和-11.68 kJ，平均值为-4.98 kJ（-1.19 kcal）。这个值非常接近零，可以认为是零，表明伴随代谢的 ΔG°'仅为分解代谢。
