Battley E H
Biotechnol Bioeng. 1979 Nov;21(11):1929-61. doi: 10.1002/bit.260211105.
A method is described for determining the free energy of formation of the cells of Saccharomyces cerevisiae (Hansen) that are formed as the result of anaerobic growth on glucose, and aerobic growth on glucose and ethanol. The method is based on the direct relationship that exists between the enthalpy changes and the free-energy changes that accompany the oxidation of 1 g cellular material formed during these growth reactions and the degree of reduction of the same material. When the results of these calculations are used together with the free energies of formation of the reactants and of other products of a given growth reaction, it becomes possible to calculate the free-energy change accompanying this reaction. These free-energy changes are in excellent agreement with those calculated by another method based on the hypothesis that the free-energy change accompanying the conversion of the substrate plus other reactants into cellular material plus other products is equal to zero.
描述了一种测定酿酒酵母（汉森氏）细胞生成自由能的方法，这些细胞是在葡萄糖上厌氧生长以及在葡萄糖和乙醇上需氧生长的结果。该方法基于以下直接关系：在这些生长反应过程中形成的1 g细胞物质氧化时伴随的焓变和自由能变与同一物质的还原程度之间存在的关系。当将这些计算结果与给定生长反应的反应物及其他产物的生成自由能一起使用时，就有可能计算该反应伴随的自由能变化。这些自由能变化与通过另一种基于底物加其他反应物转化为细胞物质加其他产物伴随的自由能变化等于零这一假设的方法计算出的结果非常一致。
