Department of Polymer Chemistry, Faculty of Enginering, Kyoto University, Sakyo-Ku, Kyoto, Japan 606-01.
Biotechnol Bioeng. 1992 Dec 5;40(10):1271-6. doi: 10.1002/bit.260401017.
In order to develop a new protein-free cell culture system, microcarriers immobilized with insulin were synthesized. For the synthesis, glass and polyacrylamide beads were treated for the introduction of amino groups on the surface, and insulin was immobilized on the surface by using several method. Anchorage-dependent cells. mouse fibroblast cells STO and fibroic sarcoma cells HSDM(1)C(1), and the anchorage-independent cells, mouse hybridoma cells SJK132-20 and RDP 45/20 were cultivated on the microcarriers immobilized with insulin. The insulin-immobilized microcarriers did not have any effect on the proliferation of the anchorage independent cells but promoted the growth of anchorage-dependent cells remarkably. The activity of immobilized insulin was larger than that of free or adsorbed insulin. The repeated use of the insulin-immobilized microcarrier was possible, and the promotion activity in the the repeated use was greater than that in the use.
为了开发一种新的无蛋白细胞培养系统，合成了固定有胰岛素的微载体。为此，对玻璃和聚丙烯酰胺珠进行了处理，以便在表面上引入氨基，并用几种方法将胰岛素固定在表面上。将贴壁依赖性细胞（小鼠成纤维细胞 STO 和纤维肉瘤细胞 HSDM(1)C(1)）和非贴壁依赖性细胞（小鼠杂交瘤细胞 SJK132-20 和 RDP 45/20）培养在固定有胰岛素的微载体上。固定有胰岛素的微载体对非贴壁依赖性细胞的增殖没有任何影响，但显著促进了贴壁依赖性细胞的生长。固定化胰岛素的活性大于游离或吸附胰岛素的活性。可以重复使用固定化胰岛素的微载体，并且在重复使用中的促进活性大于在初次使用中的促进活性。
