Ito Y, Chen G, Imanishi Y
Department of Material Chemistry, Faculty of Engineering, Kyoto University, Japan.
Biotechnol Prog. 1996 Sep-Oct;12(5):700-2. doi: 10.1021/bp9600602.
Photoreactive insulin was synthesized by coupling with azidobenzoic acid. The insulin derivative was immobilized onto the wells of polystyrene culture plates by photoir-radiation. The photoimmobilized insulin enhanced the growth of anchorage-dependent cells such as Chinese hamster ovary CHO-K1 and mouse fibroblast STO cells by more than native or azidophenyl-derivatized insulin. A small amount of photoimmobilized insulin (1-10% of the amount of the native or derivatized insulin) enhanced the growth of CHO-K1 and STO cells. In addition, the maximal mitogenic effect of the immobilized insulin was greater than that of native or derivatized insulin. Photoimmobilization could be a universal means of immobilizing growth factors onto the surface of materials devoid of chemical functional groups scaffolding growth factors and providing a new protein-free cell culture system or tissue engineering materials.
通过与叠氮苯甲酸偶联合成了光反应性胰岛素。通过光辐射将胰岛素衍生物固定在聚苯乙烯培养板的孔上。光固定化胰岛素比天然或叠氮苯基衍生化胰岛素更能促进依赖贴壁生长的细胞如中国仓鼠卵巢CHO-K1细胞和小鼠成纤维细胞STO细胞的生长。少量的光固定化胰岛素（天然或衍生化胰岛素量的1-10%）就能促进CHO-K1和STO细胞的生长。此外，固定化胰岛素的最大促有丝分裂作用大于天然或衍生化胰岛素。光固定化可能是一种将生长因子固定在缺乏化学官能团的材料表面的通用方法，这些官能团可支撑生长因子，并提供一种新的无蛋白细胞培养系统或组织工程材料。
