Department of Chemical Engineering, University of Alberta, Edmonton, Alberta, Canada.
Biotechnol Bioeng. 1992 Jul;40(3):427-31. doi: 10.1002/bit.260400312.
The kinetics of growth and antibody formation of an anti-interleukin-2 producing hybridoma line were studied in suspension culture at temperatures ranging from 34 degrees C to 39 degrees C. Flow cytometry was used to determine the effect of temperature on the cell cycle. Maximum cell density and monoclonal antibody yield were observed at 37 degrees C. The specific monoclonal antibody production rate was approximately constant throughout each batch experiment. Lower temperatures caused cells to stay longer in the G(1)-phase of the cell cycle, but temperature had only a marginal effect on the specific antibody production rate. Arresting of cells in the G(1)-phase by means of temperature was, therefore, not suited for enhanced monoclonal antibody production. Rather, antibody production for this hybridoma was directly linked to viable cell concentration.
在 34°C 至 39°C 的温度范围内，通过悬浮培养研究了抗白细胞介素-2 产生杂交瘤系的生长和抗体形成动力学。流式细胞术用于确定温度对细胞周期的影响。在 37°C 时观察到最大细胞密度和单克隆抗体产量。在每个批次实验中，单克隆抗体的特定生产速率几乎保持恒定。较低的温度导致细胞在细胞周期的 G1 期停留更长时间，但温度对特定抗体生产速率的影响很小。因此，通过温度使细胞停滞在 G1 期并不适合增强单克隆抗体的产生。相反，该杂交瘤的抗体产生直接与活细胞浓度相关。
