Pesari H, Grasso D
Department of Civil Engineering and The Environmental Research Institute, The University of Connecticut, Storrs, Connecticut 06269-3037, USA.
Biotechnol Bioeng. 1993 Jan 5;41(1):79-87. doi: 10.1002/bit.260410111.
Biodegradation of nitroglycerin (NG), an inhibitory, nongrowth substrate present in a multicomponent munition wastewater, was investigated in a pilot-scale batch reactor operated with both aerobic and anoxic cycles. A mixed culture was initially acclimated by gradual introduction of NG into influent and subsequently exposed to actual NG-laden production wastewater. System performance revealed that NG was amenable to aerobic biodegradation without adverse impact on removal efficiencies of other pollutants. Temporal NG concentration profiles indicated that an influent concentration of approximately 200 mg/L of NG was reduced to below detection limits in less than 5 h of aeration with no appreciable (<4%) biosorption. Failure of NG-acclimated cultures to utilize NG as a sole carbon source in bench-scale reactors suggested that NG behaved as a non-growth substrate and its degradation possibly occurred by cometabolism. Ethyl acetate present in the waste stream was an adequate growth substrate in terms of both biological and physicochemical properties. High concentrations of NO(3)-N, produced as a result of aerobic degradation of NG and other nitrogenous compounds of the waste, were treated in an anoxic phase. Approximately 95 mg/L of NO(3)-N was denitrified to below detection limits in 5 h of anoxia without the addition of external carbon sources. Two SRB cycle schemes with different static-fill times exhibited significant differences in treatment efficiencies.
在一个采用好氧和缺氧循环运行的中试规模间歇式反应器中，研究了多组分弹药废水中存在的抑制性非生长底物硝化甘油（NG）的生物降解情况。最初通过将NG逐渐引入进水来驯化混合培养物，随后将其暴露于实际含NG的生产废水中。系统性能表明，NG易于进行好氧生物降解，且对其他污染物的去除效率没有不利影响。NG的时间浓度曲线表明，在曝气不到5小时的情况下，进水浓度约为200 mg/L的NG降至检测限以下，且没有明显的（<4%）生物吸附。在实验室规模的反应器中，适应NG的培养物未能将NG用作唯一碳源，这表明NG表现为非生长底物，其降解可能通过共代谢发生。废水中存在的乙酸乙酯在生物学和物理化学性质方面都是合适的生长底物。由于NG和废水中其他含氮化合物的好氧降解产生的高浓度NO(3)-N在缺氧阶段进行处理。在缺氧5小时内，约95 mg/L的NO(3)-N被反硝化至检测限以下，无需添加外部碳源。两种具有不同静态填充时间的SRB循环方案在处理效率上表现出显著差异。
