Felinger A, Guiochon G
Department of Analytical Chemistry, University of Veszprém, Hungary.
J Chromatogr A. 1998 Feb 13;796(1):59-74. doi: 10.1016/s0021-9673(97)01075-3.
A comparative study of the optimization of the different modes of the preparative separation of binary mixtures by liquid chromatography is presented. Band profiles were calculated by means of the equilibrium-dispersive model of chromatography in the cases of isocratic elution, gradient elution, and displacement chromatography. The objective function to be maximized was the product of the production rate and the recovery yield. The production rate was calculated using the same definition of the cycle time in all cases. This common definition accounts for column regeneration after each run in each mode of the separation. The calculations reveal that the number of experimental parameters to be adjusted to achieve optimum separations is relatively small. The major parameters are the loading factor and the number of theoretical plates, besides the displacer concentration in displacement chromatography, or the gradient steepness in gradient elution. The relative advantages of the different modes of preparative chromatography are discussed.
本文对液相色谱法分离二元混合物不同制备分离模式的优化进行了比较研究。在等度洗脱、梯度洗脱和置换色谱的情况下，通过色谱的平衡分散模型计算了谱带轮廓。要最大化的目标函数是生产率和回收率的乘积。在所有情况下，生产率均使用相同的循环时间定义进行计算。这个通用定义考虑了每种分离模式每次运行后柱的再生。计算结果表明，为实现最佳分离而需要调整的实验参数数量相对较少。除了置换色谱中的置换剂浓度或梯度洗脱中的梯度陡度外，主要参数是负载因子和理论塔板数。讨论了制备色谱不同模式的相对优势。
