使用TLX超速离心机分离不同密度脂蛋白以及超速离心前人体血浆冻融的影响。
Use of the TLX ultracentrifuge for the isolation of different density lipoproteins and effects of freeze/thawing of human plasma before ultracentrifugation.
作者信息
Charlton-Menys Valentine, Chobotova Jelena, Durrington Paul N
机构信息
Cardiovascular Research Group, School of Clinical and Laboratory Sciences, Core Technology Facility, University of Manchester, Manchester, UK.
出版信息
Clin Chem Lab Med. 2008;46(9):1285-8. doi: 10.1515/CCLM.2008.254.
BACKGROUND
Isolation of different density lipoproteins by ultracentrifugation can require lengthy centrifugation times and freeze/thawing of plasma may influence recovery.
METHODS
We isolated a range of lipoproteins using a preparative ultracentrifuge and the TLX micro-ultracentrifuge and determined the effect of freeze/thawing of plasma beforehand.
RESULTS
In fresh plasma, there was no significant difference in results for small-dense low-density lipoprotein apolipoprotein B (LDL apoB) (density >1.044 g/mL) or cholesterol at density >1.006 g/mL. Freeze/thawing had no effect on closely correlated results for small-dense LDL apoB (r=0.85; p<0.0001) or high-density lipoprotein (r=0.93; p<0.0001).
CONCLUSIONS
The TLX micro-ultracentrifuge is a reliable alternative to the preparative ultracentrifuge and freeze/thawing has only a small effect on small-dense LDL apoB or high-density lipoprotein cholesterol.
背景
通过超速离心法分离不同密度的脂蛋白可能需要较长的离心时间，并且血浆的冻融可能会影响回收率。
方法
我们使用制备型超速离心机和TLX微型超速离心机分离了一系列脂蛋白，并预先确定了血浆冻融的影响。
结果
在新鲜血浆中，小而密低密度脂蛋白载脂蛋白B（LDL apoB）（密度>1.044 g/mL）或密度>1.006 g/mL的胆固醇的结果无显著差异。冻融对小而密LDL apoB（r=0.85；p<0.0001）或高密度脂蛋白（r=0.93；p<0.0001）的密切相关结果没有影响。
结论
TLX微型超速离心机是制备型超速离心机的可靠替代方法，冻融对小而密LDL apoB或高密度脂蛋白胆固醇只有很小的影响。
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