Charlton-Menys Valentine, Chobotova Jelena, Durrington Paul N
Cardiovascular Research Group, School of Clinical and Laboratory Sciences, Core Technology Facility, University of Manchester, Manchester, UK.
Clin Chem Lab Med. 2008;46(9):1285-8. doi: 10.1515/CCLM.2008.254.
Isolation of different density lipoproteins by ultracentrifugation can require lengthy centrifugation times and freeze/thawing of plasma may influence recovery.
We isolated a range of lipoproteins using a preparative ultracentrifuge and the TLX micro-ultracentrifuge and determined the effect of freeze/thawing of plasma beforehand.
In fresh plasma, there was no significant difference in results for small-dense low-density lipoprotein apolipoprotein B (LDL apoB) (density >1.044 g/mL) or cholesterol at density >1.006 g/mL. Freeze/thawing had no effect on closely correlated results for small-dense LDL apoB (r=0.85; p<0.0001) or high-density lipoprotein (r=0.93; p<0.0001).
The TLX micro-ultracentrifuge is a reliable alternative to the preparative ultracentrifuge and freeze/thawing has only a small effect on small-dense LDL apoB or high-density lipoprotein cholesterol.
通过超速离心法分离不同密度的脂蛋白可能需要较长的离心时间，并且血浆的冻融可能会影响回收率。
我们使用制备型超速离心机和TLX微型超速离心机分离了一系列脂蛋白，并预先确定了血浆冻融的影响。
在新鲜血浆中，小而密低密度脂蛋白载脂蛋白B（LDL apoB）（密度>1.044 g/mL）或密度>1.006 g/mL的胆固醇的结果无显著差异。冻融对小而密LDL apoB（r=0.85；p<0.0001）或高密度脂蛋白（r=0.93；p<0.0001）的密切相关结果没有影响。
TLX微型超速离心机是制备型超速离心机的可靠替代方法，冻融对小而密LDL apoB或高密度脂蛋白胆固醇只有很小的影响。
