Haig David M, Grant Dawn, Deane David, Campbell Iris, Thomson Jackie, Jepson Catherine, Buxton David, Russell George C
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, Scotland, UK.
Vaccine. 2008 Aug 18;26(35):4461-8. doi: 10.1016/j.vaccine.2008.06.056. Epub 2008 Jul 2.
The aim of this study was to stimulate immunity in the oro-nasal-pharyngeal region of cattle to protect them from alcelaphine herpesvirus-1 (AlHV-1)-induced malignant catarrhal fever. Attenuated C500 strain AlHV-1 was used along with Freund's adjuvant intramuscularly (IM) in the upper neck region to immunise cattle. Virulent C500 strain AlHV-1 was used for intranasal challenge. Nine of ten cattle were protected. Protection was associated with high levels of neutralising antibody in nasal secretions. Some protected animals showed transient low levels of viral DNA in blood samples and in one lymph node sample after challenge whereas viral DNA was detected in the blood and in lymph node samples of all animals with MCF. This is the most promising immunisation strategy to date for the control of malignant catarrhal fever.
本研究的目的是刺激牛口鼻咽部的免疫力，以保护它们免受非洲马瘟病毒1型（AlHV-1）引起的恶性卡他热。减毒的C500株AlHV-1与弗氏佐剂一起在上颈部区域进行肌肉注射（IM）以免疫牛。使用强毒C500株AlHV-1进行鼻内攻毒。十头牛中有九头受到保护。保护作用与鼻分泌物中高水平的中和抗体有关。一些受保护的动物在攻毒后血液样本和一个淋巴结样本中显示出短暂的低水平病毒DNA，而在所有患恶性卡他热的动物的血液和淋巴结样本中都检测到了病毒DNA。这是迄今为止控制恶性卡他热最有前景的免疫策略。
