Bouvrais Hélène, Méléard Philippe, Pott Tanja, Jensen Knud J, Brask Jesper, Ipsen John H
Department of Physics and Chemistry, MEMPHYS-Center for Biomembrane Physics, University of Southern Denmark, Odense, Denmark.
Biophys Chem. 2008 Sep;137(1):7-12. doi: 10.1016/j.bpc.2008.06.004. Epub 2008 Jun 17.
Magainin 2 belongs to the family of peptides, which interacts with the lipid membranes. The present work deals with the effect of this peptide on the mechanical properties of 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine Giant Unilamellar Vesicle, characterized by the bending stiffness modulus. The bending elastic modulus is measured by Vesicle Fluctuation Analysis at biologically relevant pH and physiological buffer conditions and shows a dramatic decrease with increasing peptide concentration. The observed bilayer softening is interpreted in terms of a continuum model describing perturbations on the membrane organization. Our analysis suggests that the adsorbed peptides give rise to considerable local curvature disruptions of the membrane.
马盖宁2属于肽家族,它与脂质膜相互作用。本研究探讨了该肽对1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱巨型单层囊泡力学性质的影响,该性质以弯曲刚度模量为特征。在生物学相关的pH值和生理缓冲条件下,通过囊泡涨落分析测量弯曲弹性模量,结果表明随着肽浓度的增加,模量急剧下降。观察到的双层膜软化现象,是根据描述膜组织扰动的连续介质模型来解释的。我们的分析表明,吸附的肽会引起膜的相当大的局部曲率破坏。