Miyake Mika, Ide Kazuki, Sasaki Katsunori, Matsukura Yasuko, Shijima Kumiko, Fujiwara Daisuke
Central Laboratories for Frontier Technology, Kirin Holdings Co, Ltd, Yokohama, Japan.
Biosci Biotechnol Biochem. 2008 Jul;72(7):1781-8. doi: 10.1271/bbb.80074. Epub 2008 Jul 7.
We have previously reported that highly oligomeric procyanidins (HOPC) purified from Jatoba, a South American herb, ameliorated experimental autoimmune encephalomyelitis (EAE) in mice. In this present study, we report that symptoms of arthritis were also significantly reduced by administering the Jatoba extract, when compared with the vehicle-alone-treated control. Interferon-gamma (IFN-gamma) production by the splenocytes from mice injected with procyanidins was also dramatically decreased. The oral administration of purified HOPC was significantly more effective in disease prevention than the ethanol (EtOH) extract of Jatoba. Green tea polyphenol administration, however, surprisingly facilitated disease development. Observation of the joint histopathology on whole paws derived from the HOPC-treated mice showed complete abrogation of collagen induced arthritis (CIA), a characteristic of chronic inflammation in the synovial tissue. These results demonstrate that HOPC administration had an inhibitory effect on both chronic arthritis and EAE and that the oral administration of HOPC exerted its effect after the induction of secondary immunity.
我们之前曾报道，从南美草药jatoba中纯化得到的高聚原花青素（HOPC）可改善小鼠实验性自身免疫性脑脊髓炎（EAE）。在本研究中，我们报告称，与仅用赋形剂处理的对照组相比，给予jatoba提取物后，关节炎症状也显著减轻。注射原花青素的小鼠脾细胞产生的干扰素-γ（IFN-γ）也显著减少。口服纯化的HOPC在疾病预防方面比jatoba的乙醇（EtOH）提取物更有效。然而，令人惊讶的是，给予绿茶多酚会促进疾病发展。对HOPC处理小鼠的整个爪子进行关节组织病理学观察显示，胶原诱导的关节炎（CIA）完全消失，CIA是滑膜组织慢性炎症的一个特征。这些结果表明，给予HOPC对慢性关节炎和EAE均有抑制作用，且口服HOPC在诱导二次免疫后发挥作用。
