Kamarudin Taty Anna, Othman Faizah, Mohd Ramli Elvy Suhana, Md Isa Nurismah, Das Srijit
Department of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abd Aziz, 50300 Kuala Lumpur, MALAYSIA.
EXCLI J. 2012 May 15;11:226-36. eCollection 2012.
Curcuma longa (turmeric) rhizomes contains curcumin, an active compound which possesses anti-inflammatory effects. Collagen-induced arthritis (CIA) is an accepted experimental animal model of rheumatoid arthritis. The present study aimed to observe the histological changes in the joints of experimental arthritic rats treated with curcumin. Twenty four male Sprague-Dawley (approximately 7 weeks-old) rats were randomly divided into four groups. Three groups were immunized with 150 µg collagen. All rats with established CIA, with arthritis scores exceeding 1, were orally treated with betamethasone (0.5 mg/ml/kg body weight), curcumin (110 mg/ml/kg body weight) or olive oil (1.0 ml/kg body weight) daily, for two weeks. One remaining group was kept as normal control. Treatment with 110 mg/ml/kg curcumin showed significant mean differences in the average white blood cell (WBC) count (p<0.05), cell infiltration, bone and cartilage erosion scores (p<0.05) compared to the olive oil treated group. Pannus formation scores showed that curcumin supplementation successfully suppressed the pannus formation process that occurred in the articular cartilage of the CIA joints. The mean difference for histological scores for the curcumin group was insignificant compared to the betamethasone treated group. It is concluded that supplementation of curcumin has protective effect on the histopathological and degenerative changes in the joints of CIA rats which was at par with betamethasone.
姜黄根茎含有姜黄素，这是一种具有抗炎作用的活性化合物。胶原诱导的关节炎（CIA）是一种公认的类风湿性关节炎实验动物模型。本研究旨在观察用姜黄素治疗的实验性关节炎大鼠关节的组织学变化。将24只雄性斯普拉格-道利大鼠（约7周龄）随机分为四组。三组用150μg胶原蛋白进行免疫。所有已患CIA且关节炎评分超过1的大鼠，每天口服倍他米松（0.5mg/ml/kg体重）、姜黄素（110mg/ml/kg体重）或橄榄油（1.0ml/kg体重），持续两周。其余一组作为正常对照组。与橄榄油治疗组相比，用110mg/ml/kg姜黄素治疗显示平均白细胞（WBC）计数（p<0.05）、细胞浸润、骨和软骨侵蚀评分（p<0.05）有显著差异。血管翳形成评分表明，补充姜黄素成功抑制了CIA关节软骨中发生的血管翳形成过程。与倍他米松治疗组相比，姜黄素组组织学评分的平均差异不显著。得出的结论是，补充姜黄素对CIA大鼠关节的组织病理学和退行性变化具有保护作用，与倍他米松相当。
