Lee Young-Seob, Kang Ok-Hwa, Choi Jang-Gi, Oh You-Chang, Chae Hee-Sung, Kim Jong Hak, Park Hyun, Sohn Dong Hwan, Wang Zheng-Tao, Kwon Dong-Yeul
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Jeonbuk, Republic of Korea.
J Microbiol. 2008 Jun;46(3):283-8. doi: 10.1007/s12275-008-0012-7. Epub 2008 Jul 5.
The antimicrobial killing activity toward methicillin-resistant Staphylococcus aureus (MRSA) has been a serious emerging global issue. New effective antimicrobials and/or new approaches to settle this issue are urgently needed. The oriental herb, Alpinia officinarum, has been used in Korea for several hundreds of years to treat various infectious diseases. As it is well known, one of the active constituents of Alpinia officinarum is galangin. Against the 17 strains, the minimum inhibitory concentrations (MICs) of galangin (GAL) were in the range of 62.5 ~ 125 microg/ml, and the MICs of gentamicin (GEN) ranged from 1.9 microg/ml to 2,000 microg/ml. The fractional inhibitory concentrations (FICs) of GAL, in combination with GEN, against 3 test strains were 0.4, 3.9, and 250 microg/ml, and were all 15.62 microg/ml in GEN. The FIC index showed marked synergism in the value range of 0.19 to 0.25. By determining time-kill curves, also confirmed the low synergism of the GAL and GEN combination against 4 h, 8 h, 12 h, and 24 h cultured MRSA. The time-kill study results indicated a low synergistic effect against 3 test strains. Thus, the mixture of GAL and GEN could lead to the development of new combination antibiotics against MRSA infection.
对耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌杀伤活性已成为一个严重的全球性新问题。迫切需要新的有效抗菌药物和/或解决该问题的新方法。东方草药高良姜在韩国已被用于治疗各种传染病数百年。众所周知，高良姜的活性成分之一是高良姜素。针对17株菌株，高良姜素（GAL）的最低抑菌浓度（MIC）在62.5至125微克/毫升范围内，庆大霉素（GEN）的MIC范围为1.9微克/毫升至2000微克/毫升。GAL与GEN联合对3株测试菌株的部分抑菌浓度（FIC）分别为0.4、3.9和250微克/毫升，GEN的FIC均为15.62微克/毫升。FIC指数在0.19至0.25的范围内显示出明显的协同作用。通过测定时间杀菌曲线，也证实了GAL与GEN联合对培养4小时、8小时、12小时和24小时的MRSA的协同作用较低。时间杀菌研究结果表明对3株测试菌株的协同作用较低。因此，GAL和GEN的混合物可能会导致开发出针对MRSA感染的新型联合抗生素。
