实际肝切除术后1至2小时再生大鼠肝脏中的胸苷代谢。
Thymidine metabolism in regenerating rat liver one to two hours after practical hepatectomy.
作者信息
Ord M G, Stocken L A
出版信息
Biochem J. 1973 Nov;136(3):571-7. doi: 10.1042/bj1360571.
Abstract
- When [(3)H]thymidine was injected intravenously into rats in amounts up to 40mg/kg body wt. and the (3)H radioactivity in the livers measured at 30min, saturation kinetics for thymidine uptake were not found. If the animals were examined 3 min after intravenous injection, saturation could be attained in normal rats with 12mg of thymidine/kg and in partially hepatectomized rats with 4mg/kg. At concentrations of thymidine close to saturation, no differences were found in rate or amount of uptake/g of liver between normal and partially hepatectomized rats 1-2h after operation. 2. Perfusion techniques were used to compare thymidine uptakes in the two sets of rats at concentrations up to 40mum-thymidine. Uptakes with tracer amounts of thymidine after 30min were identical in vivo and in the perfusion studies and were twice as great in livers from partially hepatectomized rats with concentrations up to 40mum-thymidine. 3. At 1.5h after operation there was nearly twice as much beta-aminoisobutyrate present per g of liver from partially hepatectomized as compared with normal rats.
摘要
- 当以高达40mg/kg体重的剂量将[³H]胸腺嘧啶核苷静脉注射到大鼠体内，并在30分钟时测量肝脏中的³H放射性时，未发现胸腺嘧啶核苷摄取的饱和动力学。如果在静脉注射后3分钟检查动物，正常大鼠给予12mg胸腺嘧啶核苷/kg、部分肝切除的大鼠给予4mg/kg时可达到饱和。在胸腺嘧啶核苷浓度接近饱和时，术后1 - 2小时正常大鼠和部分肝切除大鼠每克肝脏的摄取速率或摄取量没有差异。2. 使用灌注技术比较两组大鼠在高达40μM胸腺嘧啶核苷浓度下的胸腺嘧啶核苷摄取情况。30分钟后，示踪量胸腺嘧啶核苷的摄取在体内和灌注研究中是相同的，并且在部分肝切除大鼠的肝脏中，高达40μM胸腺嘧啶核苷浓度时的摄取量是正常大鼠的两倍。3. 术后1.5小时，部分肝切除大鼠每克肝脏中存在的β-氨基异丁酸比正常大鼠多近两倍。
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