Ogata I, Mochida S, Tomiya T, Fujiwara K
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Hepatology. 1991 Aug;14(2):361-7.
Hepatocyte contribution to hepatic collagen production in vivo was estimated in rats, based on the fact that ornithine is used for protein synthesis in the liver as arginine after conversion by way of the urea cycle only by hepatocytes. From rats given a mixture of [14C] ornithine and [3H]arginine, hepatic collagen and serum albumin were obtained. The hepatocyte contribution was calculated from the 14C and 3H in arginine purified from collagen and albumin by high performance liquid chromatography. The contribution was less than 10% of total collagen production in normal and early fibrotic livers induced by a single dose of carbon tetrachloride or dimethylnitrosamine. We conclude that hepatocytes may play a minor role in collagen production in normal and early fibrotic rat livers.
基于仅肝细胞能通过尿素循环将鸟氨酸转化为精氨酸后用于肝脏蛋白质合成这一事实，在大鼠体内评估了肝细胞对肝脏胶原蛋白生成的贡献。从给予[14C]鸟氨酸和[3H]精氨酸混合物的大鼠中获取肝脏胶原蛋白和血清白蛋白。通过高效液相色谱法从胶原蛋白和白蛋白中纯化出精氨酸，根据其中的14C和3H计算肝细胞的贡献。在单次给予四氯化碳或二甲基亚硝胺诱导的正常和早期纤维化肝脏中，该贡献占总胶原蛋白生成量的比例不到10%。我们得出结论，在正常和早期纤维化大鼠肝脏中，肝细胞在胶原蛋白生成中可能起次要作用。
