单次脉冲激光照射对色素性视网膜色素上皮（RPE）细胞的选择性靶向作用：一项体外研究。

Selective targeting of pigmented retinal pigment epithelial (RPE) cells by a single pulsed laser irradiation: an in vitro study.

作者信息

Song Q, Risco R, Latina M, Berthiaume F, Nahmias Y, Yarmush M L

机构信息

The Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School and ShrinersBurns Hospital, 51 Blossom Street, Boston, MA 02114, USA.

出版信息

Opt Express. 2008 Jul 7;16(14):10518-28. doi: 10.1364/oe.16.010518.

DOI:10.1364/oe.16.010518

PMID:18607465

Abstract

This work describes the selective targeting of pigmented retinal pigment epithelial (RPE) cells by a single pulsed laser irradiation. We observed: (1) single pulsed laser irradiation caused cellular damages on pigmented, and not on non-pigmented RPE cells at laser radiant exposure up to 2550 mJ/cm(2); (2) in the mixture of pigmented and non-pigmented RPE cells, single pulsed laser-induced damage was confined to pigmented RPE cells. This study demonstrates that the pigmented RPE cells can be selectively damaged, using a single pulsed laser irradiation, without thermal coagulation to adjacent non-pigmented RPE cells.

摘要

这项工作描述了通过单次脉冲激光照射对色素性视网膜色素上皮（RPE）细胞进行选择性靶向。我们观察到：（1）在激光辐照剂量高达2550 mJ/cm²时，单次脉冲激光照射对色素性RPE细胞造成细胞损伤，而对非色素性RPE细胞没有损伤；（2）在色素性和非色素性RPE细胞的混合物中，单次脉冲激光诱导的损伤仅限于色素性RPE细胞。本研究表明，使用单次脉冲激光照射可以选择性地损伤色素性RPE细胞，而不会对相邻的非色素性RPE细胞进行热凝固。