Hansen F G, Atlung T, Braun R E, Wright A, Hughes P, Kohiyama M
Department of Microbiology, Technical University of Denmark, Lyngby.
J Bacteriol. 1991 Aug;173(16):5194-9. doi: 10.1128/jb.173.16.5194-5199.1991.
The DnaA protein concentration was determined in five different Escherichia coli strains and in Salmonella typhimurium LT2 growing at different growth rates. The DnaA protein concentration was found to be invariant over a wide range of growth rates in the four E. coli K-12 strains and in S. typhimurium. In E. coli B/r the DnaA protein concentration was generally higher than in the K-12 strains, and it increased with decreasing growth rates. For all the strains, there appears to be a correlation between the DnaA protein concentration and the initiation mass. This supports the concept of the concentration of DnaA protein setting the initiation mass and, thus, that the DnaA protein is a key molecule in the regulation of initiation of chromosome replication in members of the family Enterobacteriaceae.
在五种不同的大肠杆菌菌株以及以不同生长速率生长的鼠伤寒沙门氏菌LT2中测定了DnaA蛋白浓度。发现在四种大肠杆菌K - 12菌株和鼠伤寒沙门氏菌中，DnaA蛋白浓度在很宽的生长速率范围内是不变的。在大肠杆菌B/r中，DnaA蛋白浓度通常高于K - 12菌株，并且随着生长速率的降低而增加。对于所有菌株，DnaA蛋白浓度与起始质量之间似乎存在相关性。这支持了DnaA蛋白浓度设定起始质量的概念，因此，DnaA蛋白是肠杆菌科成员染色体复制起始调控中的关键分子。
